bs-0295G-HRP
[Conjugated Secondary Antibody]
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated
DATASHEET
Host: Goat
Clonality: Polyclonal
Isotype: IgG
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Conjugation: HRP
Excitation/ Emission: N/A
Size: 200ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:50-200)
IHC-F(1:50-200)
Cross Reactive Species: Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Yang, et al. "Evaluation of three-dimensional scaffolds prepared from poly (3-hydroxybutyrate-< i> co</i>-3-hydroxyhexanoate) for growth of allogeneic chondrocytes for cartilage repair in rabbits." Biomaterials 29.19 (2008): 2858-2868.Read more>>
- Xu, Haixing, Yuhua Yan, and Shipu Li. "PDLLA/chondroitin sulfate/chitosan/NGF conduits for peripheral nerve regeneration." Biomaterials 32.20 (2011): 4506-4516.Read more>>
- Ma, Yanqin, et al. "Inflammatory responses induced by fluoride and arsenic at toxic concentration in rabbit aorta." Archives of toxicology 86.6 (2012): 849-856.Read more>>
- Lu, Cong-Xiao, et al. "Sulfated polymannuroguluronate, a novel anti-AIDS drug candidate, inhibits HIV-1 Tat-induced angiogenesis in Kaposi's sarcoma cells." Biochemical pharmacology 74.9 (2007): 1330-1339.Read more>>
- Liu, Hongxia, et al. "Ultrasound-Mediated Destruction of LHRHa Targeted and Paclitaxel Loaded Lipid Microbubbles Induces Proliferation Inhibition and Apoptosis in Ovarian Cancer Cells." Molecular Pharmaceutics (2013).Read more>>
- Wang, Ye-Jun, et al. "Two oral HBx vaccines delivered by live attenuated< i> Salmonella</i>: Both eliciting effective anti-tumor immunity." Cancer letters 263.1 (2008): 67-76.Read more>>
- Li, Gang, et al. "Overexpression of human papillomavirus (HPV) type 16 oncoproteins promotes angiogenesis< i> via</i> enhancing HIF-1?? and VEGF expression in non-small cell lung cancer cells."Cancer letters 311.2 (2011): 160-170..Read more>>
- Chen, Liang, et al. "Electrospun Poly (L-lactide)/Poly (??-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells."PLOS ONE?8.8 (2013): e71265..Read more>>
- Su, Bao, et al. "In Vitro and In Vivo Evaluations of Nano-Hydroxyapatite/Polyamide 66/Glass Fibre (n-HA/PA66/GF) as a Novel Bioactive Bone Screw." PLOS ONE 8.7 (2013): e68342.Read more>>
- Yan, Yu-xian, et al. "Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation." PLoS One 7.4 (2012): e35709.Read more>>
- Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012).Read more>>
- Zhang, Boce, Ariella Feldman, and Qin Wang. "A novel insight in rapid allergen detection in food systems: From threshold dose to real-world concentration."Sensors and Actuators B: Chemical?186 (2013): 597-602.Read more>>
- Yu, Tianxiao, et al. "Electrochemical immunosensor for competitive detection of neuron specific enolase using functional carbon nanotubes and gold nanoprobe." Talanta (2012).Read more>>
- He, Fengqin, Peng Yu, and Ruiyong Wu. "Relationship between sexual satiety and motivation, brain androgen receptors and testosterone in male mandarin voles." Behavioural brain research (2013).Read more>>
- Wang, J., et al. "Reinforcing properties of pups versus cocaine for fathers and associated central expression of Fos and tyrosine hydroxylase in mandarin voles (Microtus mandarinus)." Behavioural brain research 230.1 (2012): 149.Read more>>
- Wen-Qing, Hu, Peng Chun-Wei, and Li Yan. "The expression and significance of P-glycoprotein, lung resistance protein and multidrug resistance-associated protein in gastric cancer." Journal of Experimental & Clinical Cancer Research 28 (2009).Read more>>
- Zhang, Zi?\ji, et al. "iRNA expression profile during osteogenic differentiation of human adipose?\derived stem cells."Journal of cellular biochemistry 113.3 (2012): 888-898..cRead more>>
- Li, Guiying, et al. "Gremlin Aggravates Hyperglycemia?\Induced Podocyte Injury by a TGF??/Smad Dependent Signaling Pathway."ournal of cellular biochemistry?(2013).::Read more>>
- Zou, Li-Ping, et al. "Maternal IgG suppresses NMDA-induced spasms in infant rats and inhibits NMDA-mediated neurotoxicity in hippocampal neurons." Journal of neuroimmunology 181.1 (2006): 106-111.Read more>>
- Liu, Yang, et al. "A Simple Method for Isolating and Culturing the Rat Brain Microvascular Endothelial Cells." Microvascular Research (2013).Read more>>
- Jiang, An, et al. "miR-615-3p promotes the phagocytic capacity of splenic macrophages by targeting ligand-dependent nuclear receptor corepressor in cirrhosis-related portal hypertension." Experimental Biology and Medicine 236.6 (2011): 672-680.Read more>>
- Dong, Xian-Zhe, et al. "A representative prescription for emotional disease, Ding-Zhi-Xiao-Wan restores 5-HT system deficit through interfering the synthesis and transshipment in chronic mild stress-induced depressive Rats." Journal of Ethnopharmacology (2013).Read more>>
- Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013).Read more>>
- WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012).Read more>>
- Xu, Zhi-Yan, et al. "Cold-inducible expression of< i> AZI1</i> and its function in improvement of freezing tolerance of< i> Arabidopsis thaliana</i> and< i> Saccharomyces cerevisiae</i>." Journal of plant physiology 168.13 (2011): 1576-1587.Read more>>
- Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B</sub> receptors and ??< sub> 1</sub>-adrenoceptors in spontaneously hypertensive rat arteries."European journal of pharmacology (2013)..Read more>>
- Li, Yuping, et al. "Direct detection of rutin degrading isozymes with PAGE."Analytical Biochemistry (2013).Read more>>
- Gao, Qian, et al. "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." Molecular biology reports 39.5 (2012): 5791-5804.Read more>>
- Gao, Fei, and YanFen Ye. "Water bath hyperthermia reduces stemness of colon cancer cells." Clinical Biochemistry (2013).Read more>>
- Cong, Liang, et al. "Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic< i> hIGF</i>-I silkworms." Biochemical and biophysical research communications 410.4 (2011): 721-725.Read more>>
- Zhang, Feng-Lei, Hai-Qing Gao, and Lin Shen. "Inhibitory effect of GSPE on RAGE expression induced by advanced glycation end products in endothelial cells." Journal of cardiovascular pharmacology 50.4 (2007): 434-440.Read more>>
- Zhang, Wan-Dang Wang, et al. "Assessing the role of IL-35 in colorectal cancer progression and prognosis." Int J Clin Exp Pathol 6.9 (2013): 1806-1816.Read more>>
- Zhang, Wan-Dang Wang, et al. "Assessing the role of IL-35 in colorectal cancer progression and prognosis." Int J Clin Exp Pathol 6.9 (2013): 1806-1816.Read more>>
- Wang, S-J., et al. "Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)." Theriogenology 78.7 (2012): 1517-1526.Read more>>
- Ma, Song?\Tao, et al. "ffect of Arctiin on Glomerular Filtration Barrier Damage in STZ?\Induced Diabetic Nephropathy Rats."Phytotherapy Research (2012)..rRead more>>
- Ma, Song‐Tao, et al. "Effect of Arctiin on Glomerular Filtration Barrier Damage in STZ‐Induced Diabetic Nephropathy Rats." Phytotherapy Research (2012).Read more>>
- Duan, Hubin, et al. "The role of neuropeptide Y and aquaporin 4 in the pathogenesis of intestinal dysfunction caused by traumatic brain injury." Journal of Surgical Research (2013).Read more>>
- Wang, Yan, et al. "The Chronic Effects of Low Lead Level on The Expressions of Nrf2 and Mrp1 of The Testes in The Rats." Environmental Toxicology and Pharmacology (2012).Read more>>
- Fan, Tingjun, et al. "Establishment of a continuous untransfected human corneal endothelial cell line and its biocompatibility to denuded amniotic membrane." Molecular vision 17 (2011): 469.Read more>>
- Yan, Shu, et al. "Expression of endoplasmic reticulum stress-related factors in the retinas of diabetic rats." Experimental diabetes research 2012 (2011).Read more>>
- Chen, Cui-Ying, et al. "Characterization of cytotoxicity-related gene expression in response to virulent Marek’s disease virus infection in the bursa of Fabricius." Research in Veterinary Science (2012).Read more>>
- Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013).Read more>>
- Liu, Dong, and Zhong-Xiang Niu. "Cloning of a gene fragment encoding chicken complement component C3d with expression and immunogenicity of Newcastle disease virus F gene?CC3d fusion protein."Avian Pathology 37.5 (2008): 477-485.iRead more>>
- He, JianAn, et al. "The change in thickness of the solidified liquid layer rather than the immobilized mass determines the frequency response of a quartz crystal microbalance." Science China Chemistry 55.1 (2012): 175-181.Read more>>
- Wang, Chunqiang, Wei Ma, and Yuhong Su. "NF-κB Pathway Contributes to Cadmium-Induced Apoptosis of Porcine Granulosa Cells." Biological trace element research (2013): 1-8.Read more>>
- Yang, L., et al. "Transplantation of Schwann Cells Differentiated from Adipose-derived Stem Cells Modifies Reactive Gliosis after Contusion Brain Injury in Rats." Journal of International Medical Research 39.4 (2011): 1344-1357.Read more>>
- Li, Fa-Rong, et al. "Reversal effect of rosmarinic acid on multidrug resistance in SGC7901/Adr cell." Journal of Asian natural products research ahead-of-print (2013): 1-10.Read more>>
- Lu, Shuang, et al. "Preparation and characterization of the polyclonal antibody against GAR domain of microtubule actin cross-linking factor 1 (MACF1)." Afr. J. Biotechnol 10 (2011): 2156-2162.Read more>>
- Feng, Yongqiang, et al. "Combination of ketamine and xylazine exacerbates cardiac dysfunction in severely scalded rats during the shock stage." Experimental and Therapeutic Medicine (2013).Read more>>
- Imamura, Tadatsugu, et al. "Antigenic and receptor binding properties of Enterovirus 68." Journal of Virology (2013): JVI-03070.Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (bs-0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (RAT pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (bs-3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE2) Polyclonal Antibody, Unconjugated (bs-46001R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:300) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (bs-0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 bs-0295G-HRP 1:100) instructionsand DAB staining.