bs-0292R [Primary Antibody]
BSA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BSA

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 280717

Swiss Prot: P02769

Source: BSA

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 8% of all plasma zinc.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 69


Cross Reactive Species: Human
Mouse
Rat
Bovine
Goat
Others
Cow

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Cheng-Hsien, et al. "MicroRNA-328 Inhibits Renal Tubular Cell Epithelial-to-Mesenchymal Transition by Targeting the CD44 in Pressure-Induced Renal Fibrosis." PloS one 9.6 (2014): e99802.Read more>>
  • Yamazoe, Hironori, et al. "Generation of a patterned co-culture system composed of adherent cells and immobilized nonadherent cells." Acta Biomaterialia (2016).Read more>>
  • Yang, Wei, et al. "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." PeerJ 5 (2017): e3950.Read more>>
  • Zhang et al. Two dimensional barcode-inspired automatic analysis for arrayed microfluidic immunoassays. (2014) Biomicrofluidic. 7:34110Read more>>
  • Wu B et al. Effects of Adsorption of Albumin and Gamma-Globulin on the Tribological Performance of a Diamond-Like Carbon Film. Journal of Wuhan University of Technology-Mater. Sci. Ed.,2019, 34(5), 1103–1108. Read more>>
  • Na Liet al. A SERS-colorimetric dual-mode aptasensor for the detection of cancer biomarker MUC1. Anal Bioanal Chem. 2020 Sep;412(23):5707-5718.Read more>>
  • Yutaro Mori. et al. KIM-1 mediates fatty acid uptake by renal tubular cells to promote progressive diabetic kidney disease. Cell Metab. 2021 May;33:1042Read more>>
  • Wei Zhang. et al. Nanometer-Sized Boron Loaded Liposomes Containing Fe3O4 Magnetic Nanoparticles and Tributyl Borate and Anti-Albumin from Bovine Serum Antibody for Thermal Neutron Detection. Materials. 2021 Jan;14(11):304Read more>>
  • Yasunobu Asawa. et al. Carborane as an Alternative Efficient Hydrophobic Tag for Protein Degradation. Bioconjugate Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
  • Jingru Qin. et al. In-silico analysis identifies the anti-liver injury targets of diammonium glycyrrhizinate: validated in perfluorooctanoic acid-lesioned mouse model. 2022 Mar 31Read more>>
VALIDATION IMAGES

Antigen: bs-0292P, BSA, 1ug/100ul \nPrimary: bs-0292R, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in MicroplateReader by 450nm. \n


Lane 1: Human Serum lysates; Lane 2: Mouse Serum lysates; Lane 3: Rat Serum lysates; Lane 4: Goat Serum lysates; Lane 5: Cow Serum lysates probed with BSA Polyclonal Antibody, Unconjugated (bs-0292R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.