Host: Rabbit
Target Protein: PLGF
Immunogen Range: 166-221/221
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5228
Swiss Prot: P49763
Source: KLH conjugated synthetic peptide derived from human PLGF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 23
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Alessandra Zecca. et al. Intratumor Regulatory Noncytotoxic NK Cells in Patients with Hepatocellular Carcinoma. Cells-Basel. 2021 Mar;10(3):614Read more>>
"Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-PLGF Polyclonal Antibody, Unconjugated (bs-0281R) at 1:200, followed by conjugation to the secondary antibody and DAB staining\n"
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PLGF Polyclonal Antibody, Unconjugated (bs-0281R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
SW480 lysates probed with PLGF Polyclonal Antibody, Unconjugated (bs-0281R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.