bs-0279R [Primary Antibody]
VEGF Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VEGF

Immunogen Range: 27-120/232


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7422

Swiss Prot: P15692

Source: KLH conjugated synthetic peptide derived from human VEGF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 24


Cross Reactive Species: Human
Mouse
Rat
Pig
Goat
Others

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ryzhov, Sergey, et al. "Role of a2b adenosine receptors in regulation of paracrine functions of stem cell antigen 1-positive cardiac stromal cells." Journal of Pharmacology and Experimental Therapeutics 341.3 (2012): 764-774.Read more>>
  • Zeng, K.-W., et al., Sprengerinin C exerts anti-tumorigenic effects in hepatocellular carcinoma via inhibition of proliferation and angiogenesis and induction of apoptosis. Eur J Pharmacol (2013)Read more>>
  • Li, Junqin, et al. "Fatty Acid Synthase Mediates the Epithelial-Mesenchymal Transition of Breast Cancer Cells." International Journal of Biological Sciences 10.2 (2014): 171-180.Read more>>
  • Zhang, Q. L., et al. "Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of liaoning cashmere goats." Genet Mol Res 12.12 (2013): 6424-6432.Read more>>
  • Qi, Yuanyuan, et al. "Inhaled NO contributes to lung repair in piglets with acute respiratory distress syndrome via increasing circulating endothelial progenitor cells." PloS one 7.3 (2012): e33859.Read more>>
  • Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).Read more>>
  • Guo, Jian-wen, et al. "Combinatorial effects of Naomai Yihao Capsules () and vascular endothelial growth factor gene-transfected bone marrow mesenchymal stem cells on angiogenesis in cerebral ischemic tissues in rats." Journal of Traditional Chinese Medicine 32.1 (2012): 87-92.Read more>>
  • Chen et al. Circulating fibrocyte mobilization in negative pressure wound therapy. (2017) J.Cell.Mol.Me. 21:1513-1522Read more>>
  • Chen et al. Circulating fibrocyte mobilization in negative pressure wound therapy. (2017) J.Cell.Mol.Me. 21:1513-1522Read more>>
  • Yasukawa et al. Synergetic Effects of PARP Inhibitor AZD2281 and Cisplatin in Oral Squamous Cell Carcinoma in Vitro and in Vivo. (2016) Int.J.Mol.Sc. 17:272Read more>>
  • Wang et al. Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage. (2012) Neural.Regen.Res. 7:595-600Read more>>
  • Bi et al. Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice. (2017) Sci.Rep. 7:10001Read more>>
  • Chen et al. Mesenchymal stem cells and CXC chemokine receptor 4 overexpression improved the therapeutic effect on colitis via mucosa repair. (2018) Exp.Ther.Med. 16:821-829Read more>>
  • Yao Q et al.Lycium Barbarum Polysaccharides Improve Retinopathy in Diabetic Sprague-Dawley Rats.(2018)Evid Based Complement Alternat Med.Nov 15;2018:7943212. Read more>>
  • Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55.Read more>>
  • Fernandez-Bueno I et al. Reliability and reproducibility of a rodent model of choroidal neovascularization based on the subretinal injection of polyethylene glycol.Mol Vis. 2019 Mar 18;25:194-203. Read more>>
  • Varghese S et al. Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Int Immunopharmacol. 2019 May 21;73:280-292.Read more>>
  • Wang J et al. A Novel RNA Aptamer-modified Riboswitch as Chemical sensor. Analytica Chimica Acta. (2019).Read more>>
  • W Jiang et al. EFFECTS OF MESENCHYMAL STEM CELL TRANSPLANTATION INTO ISCHEMIC BILE DUCT INJURY TISSUES OF RATS ON THE EXPRESSION OF VASCULAR DENSITY. Slov Vet Res 2019: 56 (4): 183–90.Read more>>
  • Zheng W et al. An injectable thermosensitive hydrogel for sustained release of apelin-13 to enhance flap survival in rat random skin flap. J Mater Sci Mater Med. 2019 Sep 9;30(9):106. Read more>>
  • Mei F et al. Collagen peptides isolated from Salmon salar and Tilapia nilotica skin accelerate wound healing by altering cutaneous microbiome colonization via up-regulated NOD2 and BD14. Agric. Food Chem. 2020, 68, 6, 1621-1633.Read more>>
  • Sun Y et al. The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism. J Cancer Res Clin Oncol. 2020 May;146(5):1139-1152. Read more>>
  • Jiebin Li. et al. Targeted Temperature Management Suppresses Hypoxia-Inducible Factor-1 and Vascular Endothelial Growth Factor Expression in a Pig Model of Cardiac Arrest. 2021 Jan 05Read more>>
  • Guangrui Chai. et al. NLRP3 Blockade Suppresses Pro-Inflammatory and Pro-Angiogenic Cytokine Secretion in Diabetic Retinopathy. Diabet Metab Synd Ob. 2020; 13: 3047C3058Read more>>
  • Zheng Chen. et al. Glycine attenuates cerebrovascular remodeling via glycine receptor alpha 2 and vascular endothelial growth factor receptor 2 after stroke. Am J Transl Res. 2020; 12(10): 6895C6907Read more>>
  • Siddharth J. Modi. et al. Discovery of VEGFR-2 inhibitors exerting significant anticancer activity against CD44+ and CD133+ cancer stem cells (CSCs): Reversal of TGF- induced epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma. Eur J Med Chem. 2020 Dec;207:112851Read more>>
  • Yuchen Yao. et al. Seasonal expressions of VEGF and its receptors VEGFR1 and VEGFR2 in the prostate of the wild ground squirrels (Spermophilus dauricus). Eur J Histochem. 2021 Mar 24; 65(2): 3219Read more>>
  • Jin Yang. et al. Protective Effect and Potential Mechanism of the Traditional Chinese Medicine Shaoyao-Gancao Decoction on Ethanol-Induced Gastric Ulcers in Rats. EVID-BASED COMPL ALT. 2022;2022:3069089Read more>>
  • Peiyang Gu. et al. Tailorable 3DP Flexible Scaffolds with Porosification of Filaments Facilitate Cell Ingrowth and Biomineralized Deposition. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Shiou-Ling Lu. et al. VEGF-Mediated Augmentation of Autophagic and Lysosomal Activity in Endothelial Cells Defends against Intracellular Streptococcus pyogenes | mBio. MBIO. 2022 JulRead more>>
  • Tingting Guo. et al. Bone regeneration of mouse critical-sized calvarial defects with human mesenchymal stem cell sheets co-expressing BMP2 and VEGF. J DENT SCI. 2022 JulRead more>>
  • Cho-Won Kim. et al. The role of KiSS1 gene on the growth and migration of prostate cancer and the underlying molecular mechanisms. LIFE SCI. 2022 Sep;:121009Read more>>
  • ae Ju-Eun. et al. Effects of erythropoietin on osteoblast in the tooth extraction socket in mice periodontitis model. FRONT PHYSIOL. 2022 Oct;0:2117Read more>>
  • Shimaa Abdelsattar. et al. The Potential Utility of Circulating Oncofetal H19 Derived miR-675 Expression versus Tissue lncRNA-H19 Expression in Diagnosis and Prognosis of HCC in Egyptian Patients. BIOMOLECULES. 2023 Jan;13(1):3Read more>>
  • Zhu, Huaisen. et al. Ginsenoside Rg1 regulates thiram-induced chondrocytes apoptosis and angiogenesis in broiler chickens. ENVIRON SCI POLLUT R. 2022 Dec;:1-15Read more>>
  • Xiaoyu Ma. et al. Multifunctional injectable hydrogel promotes functional recovery after stroke by modulating microglial polarization, angiogenesis and neuroplasticity. CHEM ENG J. 2023 May;464:14252Read more>>
  • Feng Zhao. et al. Mechanism Repositioning Based on Integrative Pharmacology: Anti-Inflammatory Effect of Safflower in Myocardial Ischemia–Reperfusion Injury. INT J MOL SCI. 2023 Jan;24(6):5313Read more>>
  • Islam Ramiya. et al. An antioxidant ameliorates allergic airway inflammation by?inhibiting HDAC 1 via HIF-1/VEGF axis suppression in mice. SCI REP-UK. 2023 Jun;13(1):1-15Read more>>
  • Hua Liu. et al. Dose-dependent bidirectional pharmacological effects of vinorelbine-based metronomic combination chemotherapy on tumor growth and metastasis and mechanisms in melanoma mouse model. FUND CLIN PHARMACOL. 2023 JulRead more>>
  • Hongming Sun. et al. Human Cord BloodCEndothelial Progenitor Cells Alleviate Intimal Hyperplasia of Arterial Damage in a Rat Stroke Model. CELL TRANSPLANT. ;()Read more>>
  • Yue-Chao Qin. et al. Osteopontin promotes gastric cancer progression via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway. WORLD J GASTRO ONCOL. 2023 Sep 15; 15(9): 1544C1555Read more>>
  • Hua Liu. et al. Dual?directional effect of vinorelbine combined with cisplatin or fluorouracil on tumor growth and metastasis in metronomic chemotherapy in breast cancer. INT J ONCOL. 2024 Feb;64(2):1-14Read more>>
  • Liya Bai. et al. Combination Nano-Delivery Systems Remodel the Immunosuppressive Tumor Microenvironment for Metastatic Triple-Negative Breast Cancer Therapy. MOL PHARMACEUT. 2024;XXXX(XXX):XXX-XXXRead more>>
  • Ling Tang. et al. Construction of ROS-Responsive Hyaluronic Acid Modified Paclitaxel and Diosgenin Liposomes and Study on Synergistic Enhancement of Anti-Ovarian Cancer Efficacy. INT J NANOMED. 2024 04Read more>>
  • Sinan Sarolu. et al. Investigation of the Efficacy of Bevacizumab Treatment in An Experimental Rat Model of Chronic Subdural Hematoma*. WORLD NEUROSURG. 2024 JuRead more>>
  • Jiajun Hu. et al. Arginine-loaded globular BSAMA/fibrous GelMA biohybrid cryogels with multifunctional features and enhanced healing for soft gingival tissue regeneration. INT J BIOL MACROMOL. 2024 Aug;:134932Read more>>
  • Wu Tao. et al. Exploring the Mechanism of Myrrh in the Treatment of Breast Cancer Based on Network Pharmacology and Cell Experiments. CHEM BIOL DRUG DES. 2024 Aug;104(2):e14604Read more>>
  • Xiaoxin Xu. et al. m6A modification of VEGFA mRNA by RBM15/YTHDF2/IGF2BP3 contributes to angiogenesis of hepatocellular carcinoma. MOL CARCINOGEN. 2024 AugRead more>>
VALIDATION IMAGES

Human colon carcinoma lysates probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 23,46kDa


Formalin-fixed and paraffin embedded: human ovary carcinoma labeled with Anti-VEGF Polyclonal Antibody, Unconjugated(bs-0279R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


HeLa cells probed with Rabbit Anti-VEGF Polyclonal Antibody (bs-0279R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated.\n


THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF Antibody(bs-0279R-AF488)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).