bs-0244R
[Primary Antibody]
HSP70 1A/1B Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: HSP70 1A/1B
Immunogen Range: 500-600/641
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3303
Swiss Prot: P08107
Source: KLH conjugated synthetic peptide derived from human HSP70 1A/1B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Mouse
Rat
Pig
Chicken
Predicted Cross Reactive Species:
Cow
Sheep
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.Read more>>
- Oyagbemi AA et al. Quercetin attenuates hypertension induced by sodium fluoride via reduction in oxidative stress and modulation of HSP 70/ERK/PPARγ signaling pathways. (2018) Sep 01Read more>>
- Zhou M et al. Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate. J Anim Physiol Anim Nutr (Berl). 2019 Sep 4. Read more>>
- Wang Y et al. Excessive Cu2+ deteriorates arsenite-induced apoptosis in chicken brain and resulting in immunosuppression, not in homeostasis. Chemosphere. 2019 Sep 4;239:124758. Read more>>
- Ling Li. et al. Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton. J Vet Sci. 2020 May; 21(3): e49Read more>>
- Xinglin Liu. et al. Effects of Dietary Zearalenone Exposure on the Growth Performance, Small Intestine Disaccharidase, and Antioxidant Activities of Weaned Gilts. Animals-Basel. 2020 Nov;10(11):2157Read more>>
- Xia Wang. et al. Distinct expression and localization patterns of HSP70 in developmental reproductive organs of rams. Gene. 2020 Nov;760:145029Read more>>
- Yanan Hu. et al. Preparation of photothermal responsive and ROS generative gold nanocages for cancer therapy. Chem Eng J. 2021 Oct;421:129744Read more>>
- Dahan Yang. et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus. Front Vet Sci. 2021; 8: 666512Read more>>
- Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692Read more>>
- Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26Read more>>
- Hong-Mei Li. et al. Anti-Cancer Properties of Ginkgolic Acids in Human Nasopharyngeal Carcinoma CNE-2Z Cells via Inhibition of Heat Shock Protein 90. Molecules. 2021 Jan;26(21):6575Read more>>
- Wang, Rui. et al. Protective Effect of Manganese on Apoptosis and Mitochondrial Function of Heat-Stressed Primary Chick Embryonic Myocardial Cells. 2021 Nov 15Read more>>
- Lee Jisu. et al. Alpha-2-macroglobulin as a novel diagnostic biomarker for human bladder cancer in urinary extracellular vesicles. FRONT ONCOL. 2022 Sep;0:4727Read more>>
- Hehe Du. et al. Cromileptes altivelis microRNA Transcriptome Analysis upon Nervous Necrosis Virus (NNV) Infection and the Effect of cal-miR-155 on Cells Apoptosis and Virus Replication. VIRUSES-BASEL. 2022 Oct;14(10):2184Read more>>
- Axel Berg-Larsen. et al. Tumor growth inhibition and immune system activation following treatment with thorium-227 conjugates and PD-1 check-point inhibition in the MC-38 murine model.. FRONT MED-LAUSANNE. 2022 Nov;9:1033303-1033303Read more>>
- Long Yuan. et al. Lactobacillus johnsonii N5 from heat stress-resistant pigs improves gut mucosal immunity and barrier in dextran sodium sulfate-induced colitis. ANIM NUTR. 2023 AugRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-HSP70/HSPA1A Polyclonal Antibody, Unconjugated (bs-0244R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with HSP70 1A/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSP70 Antibody(bs-0244R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Testis lysates; Lane 2: Mouse Epididymis lysates; Lane 3: Mouse Seminal vesicle lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse NIH/3T3 cell lysates; Lane 6: Rat Testis lysates; Lane 7: Rat Spleen lysates; Lane 8: Human Hela cell lysates; Lane 9: Human Jurkat cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human A549 cell lysates; Lane 12: Human MCF-7 cell lysates probed with HSP70 1A/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.