Host: Rabbit
Target Protein: NMDAR2B
Immunogen Range: 451-550/1484
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2904
Swiss Prot: Q13224
Source: KLH conjugated synthetic peptide derived from human NMDAR2B
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 163
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: : rat brain tissue labeled with Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37°C and DAPI staining
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37°C
A549 lysates probed with NMDAR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NR2B Polyclonal Antibody, Unconjugated (bs-0222R) at 1:200 overnight at 4°C, DAB staining.