bs-0217R
[Primary Antibody]
FGF2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: FGF2
Immunogen Range: 143-250/288
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 14173
Swiss Prot: P09038
Source: KLH conjugated synthetic peptide derived from mouse FGF2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 18
Cross Reactive Species:
Human
Mouse
Rat
Sheep
Rabbit
Predicted Cross Reactive Species:
Cow
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Pan, Ruo-Lang, et al. "Delta-like 1 serves as a new target and contributor to liver fibrosis down-regulated by mesenchymal stem cell transplantation." Journal of Biological Chemistry 286.14 (2011): 12340-12348.Read more>>
- Liu, Kai-Jun, et al. "Analysis of olfactory ensheathing glia transplantation-induced repair of spinal cord injury by electrophysiological, behavioral, and histochemical methods in rats." Journal of molecular neuroscience 41.1 (2010): 25-29.Read more>>
- Chen, Bo-Yu, et al. "Altered TGF-β2 and bFGF expression in scleral desmocytes from an experimentally-induced myopia guinea pig model." Graefes Archive for Clinical and Experimental Ophthalmology (2013): 1-12.Read more>>
- Zhao, Zhan‐Zheng, et al. "Effects of recombinant human endostatin on peritoneal angiogenesis in peritoneal dialysis rats." Nephrology 16.6 (2011): 599-606.Read more>>
- Gao, Dan, et al. "Effect of peritoneal dialysis on expression of vascular endothelial growth factor, basic fibroblast growth factor and endostatin of the peritoneum in peritoneal dialysis patients." Nephrology 16.8 (2011): 736-742. Read more>>
- Lee, Hye Sook, et al. "Anti-neovascular effect of chondrocyte-derived extracellular matrix on corneal alkaline burns in rabbits." Graefes Archive for Clinical and Experimental Ophthalmology (2014): 1-11.Read more>>
- Turgut, Burak, et al. "Topical infliximab for the suppression of wound healing following experimental glaucoma filtration surgery." Drug Design, Development and Therapy 8 (2014): 421-429.Read more>>
- Turgut, Burak, et al. "Impact of trastuzumab on wound healing in experimental glaucoma surgery." Clinical & Experimental Ophthalmology (2014).Read more>>
- Sun, Fei, et al. "Structural integrity, immunogenicity and biomechanical evaluation of rabbit decelluarized tracheal matrix." Journal of Biomedical Materials Research Part A (2014).Read more>>
- Shkurupy, V. A., et al. "In Vitro Effects of Nanosized Diamond Particles on Macrophages." Bulletin of Experimental Biology and Medicine (2015): 1-4.Read more>>
- He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015).Read more>>
- Eren, Kenan, et al. "The Suppression of Wound Healing Response with Sirolimus and Sunitinib Following Experimental Trabeculectomy in a Rabbit Model." Current Eye Research (2016): 1-10.Read more>>
- Wang et al. VEGF and BFGF Expression and Histological Characteristics of the Bone-Tendon Junction during Acute Injury Healing. (2014) J.Sports.Sci.Me. 13:15-21Read more>>
- Liu et al. B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model. (2018) Eye.(Lond). Read more>>
- Peng W et al.Expression of osteoprotegerin and receptor activator for the nuclear factor-κB ligand in XACB/LV-bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis.(2018) J. Cell. Biochem. Oct 18Read more>>
- H Mu et al. HCMV-encoded IE2 induces anxiety-depression and cognitive impairment in UL122 genetically-modified mice. Int J Clin Exp Pathol 2019;12(11):4087-4095.Read more>>
- Tort S et al. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns. 2019 Dec 17. pii: S0305-4179(18)30976-8.Read more>>
- Yan L et al. Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity. Cell Biology and Toxicology. Read more>>
- Mei F et al. Collagen peptides isolated from Salmon salar and Tilapia nilotica skin accelerate wound healing by altering cutaneous microbiome colonization via up-regulated NOD2 and BD14. Agric. Food Chem. 2020, 68, 6, 1621-1633.Read more>>
- "Gao XX et al. Effects of L-arginine on endometrial microvessel density in nutrient-restricted Hu sheep.Theriogenology. 2018 Oct 1;119:252-258. "Read more>>
- YING-HAO HAN. et al. Peroxiredoxin II Inhibits Alcohol-induced Apoptosis in L02 Hepatocytes Through AKT/-Catenin Signaling Pathway. Anticancer Res. 2020 Aug;40(8):4491-4504Read more>>
- De Nan. et al. In Vitro Study of Adipose-Derived Mesenchymal Stem Cells Transduced with Lentiviral Vector Carrying the Brain-Derived Neurotrophic Factor Gene. Int J Stem Cells. 2020; 13(3): 386C393Read more>>
- Liuhong Liu. et al. Experimental study on the effect of chrysin on skin injury induced by amiodarone extravasation in rats. Microvasc Res. 2022 Jan;139:104257Read more>>
- Ali Bagherian. et al. Anti-glioblastoma effects of nanomicelle-curcumin plus erlotinib. Food Funct. 2021 OcRead more>>
- Wenji Hu. et al. Structural characterization of polysaccharide purified from Amanita caesarea and its pharmacological basis for application in Alzheimer's disease: endoplasmic reticulum stress. Food Funct. 2021 OcRead more>>
- Liu, Jingyu. et al. Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder. Stem Cell Res Ther. 2022 Dec;13(1):1-16Read more>>
- Xiangfeng Li. et al. Design of macropore structure and micro-nano topography to promote the early neovascularization and osteoinductivity of biphasic calcium phosphate bioceramics. Mater Design. 2022 Apr;216:110581Read more>>
- Zhou, Liuhua. et al. Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats. Sci Rep-Uk. 2022 Mar;12(1):1-1Read more>>
- Fengchun Liao. et al. ECFC-derived exosomal THBS1 mediates angiogenesis and osteogenesis in distraction osteogenesis via the PI3K/AKT/ERK pathway. J ORTHOP TRANSL. 2022 Nov;37:12Read more>>
- Anna Krzy?ewska. et al. Cannabidiol alleviates right ventricular fibrosis by inhibiting the transforming growth factor pathway in monocrotaline-induced pulmonary hypertension in rats. BBA-MOL BASIS DIS. 2023 Aug;1869:166753Read more>>
- Jussi Herman Kasurinen. et al. Prognostic Values of Tissue and Serum Angiogenic Growth Factors Depend on the Phenotypic Subtypes of Colorectal Cancer. CANCERS. 2023 Jan;15(15):3871Read more>>
- Hao Zhang. et al. Dietary rumen-protected L-arginine or N-carbamylglutamate enhances placental amino acid transport and suppresses angiogenesis and steroid anabolism in underfed pregnant ewes. ANIM NUTR. 2023 AugRead more>>
- Jialing Sun. et al. Investigating the molecular mechanism of Qizhu anticancer prescription in inhibiting hepatocellular carcinoma based on high-resolution mass spectrometry and network pharmacology. J ETHNOPHARMACOL. 2024 Feb;:117985Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-BFGF/FGF2 Polyclonal Antibody, Unconjugated (bs-0217R) followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-bFGF Polyclonal Antibody (bs-0217R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
HepG2 lysates probed with bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse spleen lysates probed with bFGF Polyclonal Antibody, Unconjugated (bs-0217R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with bFGF Antibody(bs-0127R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).