Host: Rabbit
Target Protein: CD95
Immunogen Range: 35-110/327
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 14102
Swiss Prot: P25446
Source: KLH conjugated synthetic peptide derived from mouse FAS
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 40-50
Cross Reactive Species:
Human
Mouse
Rat
Monkey
For research use only. Not intended for diagnostic or therapeutic use.
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- Ewelina Dobosz. et al. Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation. Dis Model Mech. 2021 Mar;14(3)Read more>>
- Yoji Komiya. et al. Necroptosis in alveolar epithelial cells drives lung inflammation and injury caused by SARS-CoV-2 infection. BBA-MOL BASIS DIS. 2024 Dec;1870:167472Read more>>
Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Fas Polyclonal Antibody, Unconjugated (bs-0215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus tissue lysates; Lane 2: Rat Thymus tissue lysates; Lane 3: Human HepG2 cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human HT1080 cell Lysates; Lane 6: Human A431 cell Lysates probed with CD95/FAS Polyclonal Antibody, Unconjugated (bs-0215R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Raji cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD95/FAS Polyclonal Antibody(bs-0215R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).