Host: Rabbit
Target Protein: GDNFRA
Specificity: This antibody exhibits a secondary cross-reactivity towards Exonuclease-1 due to a 60% contigueous sequence similarity over the immunogen range.
Immunogen Range: 351-429/465
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2674
Swiss Prot: P56159
Source: KLH conjugated synthetic peptide derived from human GFR Alpha-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 44
Cross Reactive Species:
Human
Mouse
Goat
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Ma, Fanglin, et al. "Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT." Scientific Reports 6 (2016): 38805.Read more>>
- Li, Simin, et al. "Quantitative analysis of receptor tyrosine kinase-effector coupling at functionally relevant stimulus levels." Journal of Biological Chemistry 290.16 (2015): 10018-10036.Read more>>
- Lin Liu. et al. Aberrantly expressed GFR-1/RET in patients with lacrimal adenoid cystic carcinoma is associated with high recurrence risk: a retrospective study of 51 LACC cases. Cancer Biol Med. 2021 Feb 15; 18(1): 199C205Read more>>
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GDNFRA Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with GDNFRA Polyclonal Antibody, Unconjugated (bs-0201R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MOLT4 cell lysates; Lane 4: Human A549 cell lysates probed with GDNFRA Polyclonal Antibody, Unconjugated (bs-0201R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.