bs-0199R-FITC
[Conjugated Primary Antibody]
GFAP Polyclonal Antibody, FITC Conjugated
DATASHEET
Host: Rabbit
Target Protein: GFAP
Immunogen Range: 51-150/432
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2670
Swiss Prot: P14136
Source: KLH conjugated synthetic peptide derived from human GFAP
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Conjugation: FITC
Excitation/ Emission: 494nm/518nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 48
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao, Hongyu, et al. "Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration." The Journal of Cell Biology (2013).Read more>>
- Zhao, Yan G., et al. "The p53-induced gene Ei24 is an essential component of the basal autophagy pathway." Journal of Biological Chemistry 287.50 (2012): 42053-42063.Read more>>
- Xiang, Yanxiao, et al. "Anti-inflammatory Effect of Acetylpuerarin on Eicosanoid Signaling Pathway in Primary Rat Astrocytes."Journal of Molecular Neuroscience(2013): 1-9Read more>>
- Hu, Minghui, et al. "Neuroprotection effect of Interleukin?\17 secreted by reactive astrocytes is emerged from a high?\level IL?\17?\containing environment during acute neuroinflammation."nical & Experimental Immunology (2013).66..Read more>>
- Liu, Yang, et al. "A Simple Method for Isolating and Culturing the Rat Brain Microvascular Endothelial Cells." Microvascular Research (2013).Read more>>
- Xiang, Yanxiao, et al. "Anti-inflammatory Effect of Acetylpuerarin on Eicosanoid Signaling Pathway in Primary Rat Astrocytes."Journal of Molecular Neuroscience(2013): 1-9Read more>>
- Fan, Lixing, et al. "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." In Vitro Cellular & Developmental Biology-Animal (2013): 1-9.Read more>>
- Zuo, Daiying, et al. "Existence of glia mitigated ketamine-induced neurotoxicity in neuron-glia mixed cultures of neonatal rat cortex and the glia-mediated protective effect of 2-PMPA." Neurotoxicology (2014).Read more>>
- Ma, Benyu, et al. "Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation." Cell Research (2014).Read more>>
- Shan, Chun-Lei, et al. "High Efficiency Intracellular Transport of Cationic Peptide Stearate for Gene Delivery in Tumor Cells and Multipotent Stem Cells." Journal of Biomedical Nanotechnology 10.11 (2014): 3231-3243.Read more>>
- Du, Wenzhong, et al. "Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness." Neuro-Oncology (2014): nou217.Read more>>
- Zhao, Yan G., et al. "The autophagy gene Wdr45/Wipi4 regulates learning and memory function and axonal homeostasis." Autophagy (2015).Read more>>
- Mori, Miki, et al. "Stromal Cell-Derived Factor-1α Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." International Journal of Molecular Sciences 16.8 (2015): 18018-18032.Read more>>
- Sweeney, Patrick, et al. "Activation of hypothalamic astrocytes suppresses feeding without altering emotional states." Glia (2016).Read more>>
- Yan, Yu-hui, et al. "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." Neurochemical Research (2016): 1-8.Read more>>
- Liao, Wei-Tao, et al. "The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation." (2016).Read more>>
- Song, Eric, et al. "Surface chemistry governs cellular tropism of nanoparticles in the brain." Nature Communications (2017)Read more>>
- Hu. et al. Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 PathwayRead more>>
- Liang et al. Scorpion ethanol extract and valproic acid effects on hippocampal glial fibrillary acidic protein expression in a rat model of chronic-kindling epilepsy induced by lithium chloride-pilocarpine. (2012) Neural.Regen.Re. 7:426-33Read more>>
- Farah et al. Tau accumulations in the brains of woodpeckers. (2018) PLoS.One. 13:e0191526Read more>>
- Liao et al. The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation. (2018) Arch.Med.Sci. 14:370-380Read more>>
- Zhang,et al.Interferon-γ Promotes Neuronal Repair by Transplanted Neural Stem Cells in Ischemic Rats.(2018) Stem Cells and Development. 27:355-366.Read more>>
- Esaki,et al.ASC amino acid transporter 2, defined by enzyme-mediated activation of radical sources, enhances malignancy of GD2-positive small-cell lung cancer.(2018) Cancer Science. 109:141-153.Read more>>
- Zhong,et al.Protective effect of adenovirus-mediated erythropoietin expression on the spiral ganglion neurons in the rat inner ear.(2018) International Journal of Molecular Medicine. 41:2669-2677.Read more>>
- Zhang X et al.Neuroprotective effects of matrix metalloproteinases in cerebral ischemic rats by promoting activation and migration of astrocytes and microglia.(2018) Brain Res Bull. Nov 13.Read more>>
- Lu et al. Autophagy activator promotes neuronal differentiation of adult adipose-derived stromal cells. (2013) Neural.Regen.Res. 8:882-9Read more>>
- Yao Q et al.Lycium Barbarum Polysaccharides Improve Retinopathy in Diabetic Sprague-Dawley Rats.(2018)Evid Based Complement Alternat Med.Nov 15;2018:7943212. Read more>>
- Xiang Y et al. Inhibition of sPLA. sub. 2-IIA Prevents LPS-Induced Neuroinflammation by Suppressing ERK1/2-cPLA. sub. 2 [alpha] Pathway in Mice Cerebral Cortex.PLoS One. 2013 Oct 9;8(10):e77909. Read more>>
- Iwasa T et al. IL-10 and CXCL2 in trigeminal ganglia in neuropathic pain.Neurosci Lett. 2019 Jun 11;703:132-138. Read more>>
- H Mu et al. HCMV-encoded IE2 induces anxiety-depression and cognitive impairment in UL122 genetically-modified mice. Int J Clin Exp Pathol 2019;12(11):4087-4095.Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- Chen J et al. Modulation of activated astrocytes in the hypothalamus paraventricular nucleus to prevent ventricular arrhythmia complicating acute myocardial infarction. Int J Cardiol. 2020 Jun 1;308:33-41.Read more>>
- Kong F et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-B signaling in Alzheimer's disease. J Neuroinflammation. 2020 Oct 15;17(1):305.Read more>>
- Tian L et al. Blockade of adenosine A1 receptor in nucleus tractus solitarius attenuates baroreflex sensitivity response to dexmedetomidine in rats. Brain Res. 2020 Sep 15;1743:146949.Read more>>
- Yu D et al. MOF-encapsulated nanozyme enhanced siRNA combo: Control neural stem cell differentiation and ameliorate cognitive impairments in Alzheimer's disease model. Biomaterials. 2020 Oct;255:120160.Read more>>
- Dingkang Xu. et al. Sphingosine\1\phosphate receptor 3 is implicated in BBB injury via the CCL2\CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28Read more>>
- Xue Wang. et al. Engineered liposomes targeting the gutCCNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:39Read more>>
- Zhenting Huang . et al. Anti-Cognitive Decline by Yinxing-Mihuan-Oral-Liquid via Activating CREB/BDNF Signaling and Inhibiting Neuroinflammatory Process. 2021 Jan 27Read more>>
- Gui-Long Zhang. et al. Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis. World J Stem Cells. 2020 Nov 26; 12(11): 1396C1409Read more>>
- Fange Kong. et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-B signaling in Alzheimers disease. J Neuroinflamm. 2020 Dec;17(1):1-15Read more>>
- Nan Wang. et al. Hashimoto's Thyroiditis Induces Hippocampus-Dependent Cognitive Alterations by Impairing Astrocytes in Euthyroid Mice. 2020 Oct 12Read more>>
- Miao Zhao. et al. The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinsons disease. Cell Death Differ. 2021 Mar;:1-19Read more>>
- Mikin R. Patel. et al. Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF- signaling. Cell Rep. 2021 Mar;34:108829Read more>>
- Wei Tang. et al. Macrophage membrane-mediated targeted drug delivery for treatment of spinal cord injury regardless of the macrophage polarization states. Asian J Pharm Sci. 2021 ApRead more>>
- Min Ai. et al. 1,25(OH)2D3 attenuates sleep disturbance in mouse models of Lewis lung cancer, in silico and in vivo. 2021 Jun 01Read more>>
- Zhixiong Li. et al. Recovery of post-stroke cognitive and motor deficiencies by Shuxuening injection via regulating hippocampal BDNF-mediated Neurotrophin/Trk Signaling. Biomed Pharmacother. 2021 Sep;141:111828Read more>>
- Li Gao. et al. Baicalein Delays H2O2-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-B Pathway, and Regulation of Leucine Metabolism. Acs Chem Neurosci. 2021;12(13):2320C2335Read more>>
- Yanjing Zhu. et al. Deep learning-based predictive identification of neural stem cell differentiation. Nat Commun. 2021 May;12(1):1-13Read more>>
- ing-Chun Liu. et al. Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells. Int J Mol Sci. 2021 Jan;22(14):7473Read more>>
VALIDATION IMAGES
Hela cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GFAP Polyclonal Antibody(bs-0199R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).