bs-0197R [Primary Antibody]
VPAC2R Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VPAC2R

Immunogen Range: 81-170/437


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 29555

Swiss Prot: P35000

Source: KLH conjugated synthetic peptide derived from rat VIP receptor II

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Flow-Cyt(Flow-Cyt=2ug/Test)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ruan, Ming, et al. "Attenuation of stress-induced gastrointestinal motility disorder by gentiopicroside, from Gentiana macrophylla Pall." Fitoterapia(2015).Read more>>
  • Ye D et al. Spatiotemporal Expression Changes of PACAP and Its Receptors in Retinal Ganglion Cells After Optic Nerve Crush.(2018)J Mol Neurosci. Nov 10.Read more>>
  • Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti VIP receptor 2/VPAC2 Polyclonal Antibody, Unconjugated (bs-0197R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


A431 lysates probed with VPAC2R Polyclonal Antibody, Unconjugated (bs-0197R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Recombinant human VIPR2 protein, N-Trx-His(bs-42334P) probed with VIPR2 Polyclonal Antibody, Unconjugated (bs-0197R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.