Host: Rabbit
Target Protein: HSP60
Immunogen Range: 501-573/573
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3329
Swiss Prot: P10809
Source: KLH conjugated synthetic peptide derived from human HSP60
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 58
Cross Reactive Species:
Human
Mouse
Rat
Pig
Predicted Cross Reactive Species:
Dog
Cow
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Kengkoom, Kanchana, and Sumate Ampawong. "Chronic Ingestion of High Dosed Phikud Navakot Extraction Induces Mesangiolysis in Rats with Alteration of AQP1 and Hsp60 Expressions." BioMed Research International 2015 (2015).Read more>>
- Isarangkul, Duangnate, et al. "Mitochondrial and cytoskeletal alterations are involved in the pathogenesis of hydronephrosis in ICR/Mlac-hydro mice." International Journal of Clinical and Experimental Medicine 8.6 (2015): 9192-9204.Read more>>
- Liu et al. Autophagy induced by DAMPs facilitates the inflammation response in lungs undergoing ischemia-reperfusion injury through promoting TRAF6 ubiquitination. (2017) Cell.Death.Diffe. 24:683-693Read more>>
- Qi et al. Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma. (2008) Int.J.Cancer. 122:2682-8Read more>>
- Xinrui Zhang. et al. Wan-Nian-Qing, a Herbal Composite Prescription, Suppresses the Progression of Liver Cancer in Mice by Regulating Immune Response. Front Oncol. 2021; 11: 696282Read more>>
- Yuliang Wen. et al. Effect of glycolysis and heat shock proteins on hypoxia adaptation of Tibetan sheep at different altitude. Gene. 2021 Nov;803:145893Read more>>
- Shulipan Mulati. et al. 6-Shogaol Exhibits a Promoting Effect with Tax via Binding HSP60 in Non-Small-Cell Lung Cancer. CELLS-BASEL. 2022 Jan;11(22):3678Read more>>
- Mu?oz Vitor Rosetto. et al. Effects of short-term endurance and strength exercise in the molecular regulation of skeletal muscle in hyperinsulinemic and hyperglycemic Slc2a4+/? mice. CELL MOL LIFE SCI. 2023 May;80(5):1-16Read more>>
- Weiyi Zhang. et al. Cinnamaldehyde induces apoptosis and enhances anti-colorectal cancer activity via covalent binding to HSPD1. PHYTOTHER RES. 2023 ApRead more>>
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CYP5A1/Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse kidney lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
Mouse heart lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.
MCF-7 Lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Lane 1: Mouse Liver lysates; Lane 2: Mouse Adrenal gland lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Rat Adrenal gland lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human MDA-MB-231 cell lysates; Lane 7: Human HepG2 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human K562 cell lysates probed with HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (bs-0191R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.