Host: Rabbit
Target Protein: Preproendothelin 1
Immunogen Range: 16-110/212
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1906
Swiss Prot: P05305
Source: KLH conjugated synthetic peptide derived from human Preproendothelin 1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 22
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-ET-1 Polyclonal Antibody (bs-0188R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat stomach labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat heart muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Big endothelin-1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: mouse liver lysates; Lane 2: rat liver lysates probed with Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates probed with Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.