bs-0186R [Primary Antibody]
IRS-3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IRS-3

Immunogen Range: 261-360/494


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84021

Source: KLH conjugated synthetic peptide derived from rat IRS-3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The family of insulin receptor substrates (IRSs) has been reported to play important roles for signal transduction of various hormones. Four members of the IRS family have been described. Each IRS is believed to have different functions; however, the distinct physiological roles of each IRS are unclear. IRS-1 may mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yanhan Ma. et al. Effect of Interleukin-1 on Gene Expression Signatures in Schwann Cells Associated with Neuropathic Pain. 2021 Jul 15Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung inflammation labeled with Rabbit Anti IRS-3 Polyclonal Antibody, Unconjugated (bs-0186R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Rat adipose lysates probed with IRS-3 Polyclonal Antibody, Unconjugated (bs-0186R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.