DATASHEET
Host:
Rabbit
Target Protein:
PDGF B
Immunogen Range:
155-210/241
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
5155
Swiss Prot:
P01127
Source:
KLH conjugated synthetic peptide derived from human PDGF-B
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
PRODUCT SPECIFIC PUBLICATIONS
- Lee, Si‐Hyung, et al. "Therapeutic efficacy of autologous platelet‐rich plasma and polydeoxyribonucleotide on female pattern hair loss." Wound Repair and Regeneration (2014).Read more>>
- Morita, Shoko, et al. "Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain." Cell and Tissue Research (2015): 1-20.Read more>>
- Hurley, Marja M., et al. "Accelerated Fracture Healing in Transgenic Mice Overexpressing an Anabolic Isoform of Fibroblast Growth Factor 2." Journal of Cellular Biochemistry (2015).Read more>>
- He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015).Read more>>
- Furube et al. VEGF-dependent and PDGF-dependent dynamic neurovascular reconstruction in the neurohypophysis of adult mice. (2014) J.Endocrino. 222:161-79Read more>>
- Almahrog et al. In vivo association of immunophenotyped macrophages expressing CD163 with PDGF-B in gingival overgrowth-induced by three different categories of medications. (2016) J.Oral.Biol.Craniofac.Res. 6:10-7Read more>>
- Wang et al. Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model. (2017) PLoS.One. 12:e0178619Read more>>
- Haolin Chen. et al. Exploring the Molecular Characteristics and Role of PDGFB in Testis and Epididymis Development of Tibetan Sheep. VET SCI. 2024 Jun;11(6):266Read more>>
- Lina Dong. et al. Oriented cellulose hydrogel: Directed tissue regeneration for reducing corneal leukoplakia and managing fungal corneal ulcers. BIOACT MATER. 2024 Nov;41:15Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-PDGF-B Polyclonal Antibody, Unconjugated (bs-0185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-PDGF-B Polyclonal Antibody, Unconjugated (bs-0185R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Mouse Lung lysates; Lane 5: Rat Lung lysates; Lane 6: Human HUVEC cell lysates; Lane 7: Human A431 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human U-2OS cell lysates probed with PDGF B Polyclonal Antibody, Unconjugated (bs-0185R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.