bs-0180R [Primary Antibody]
DOPA Decarboxylase Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DOPA Decarboxylase

Immunogen Range: 201-300/480


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1644

Swiss Prot: P20711

Source: KLH conjugated synthetic peptide derived from human DDC

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hiramoto, Keiichi, Yurika Yamate, and Shosuke Kawanishi. "Detection of Dopa‐positive cells in mouse ovaries in response to ocular exposure to ultraviolet B rays." Photodermatology, Photoimmunology & Photomedicine (2014).Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti DDC/DOPA Decarboxylase Polyclonal Antibody, Unconjugated (bs-0180R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse adrenal gland tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat adrenal gland tissue lysates ; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human A549 cell lysates probed with DOPA Decarboxylase Polyclonal Antibody,Unconjugated (bs-0180R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.