bs-0178R
[Primary Antibody]
Nurr1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Nurr1
Specificity: 90% sequence similarity with Nuclear receptor subfamily 4 group A member 3
Immunogen Range: 301-400/598
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4929
Source: KLH conjugated synthetic peptide derived from human Nurr1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Nuclear receptor and transcription factor; plays a role in development and maintenance of neurons synthesizing the neurotransmitter dopamine This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full length nature has not been determined.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 67
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Qiu Qian. et al. Propofol protects H9C2 cells against hypoxia/reoxygenation injury through miR?449a and NR4A2. Exp Ther Med. 2021 Oct;22(4):1-11Read more>>
- Lijun Dai. et al. The Distribution, Expression Patterns and Functional Analysis of NR1D1 and NR4A2 in the Reproductive Axis Tissues of the Male Tianzhu White Yak. Animals-Basel. 2021 Nov;11(11):3117Read more>>
VALIDATION IMAGES
Frozen mouse hippocampus tissue labeled with Anti-Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) followed by conjugation to the secondary antibody
Lane 1: Human Hela cell lysates; Lane 2: Human U-87MG cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human Raji cell lysates; Lane 6: Human U251 cell lysates probed with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-0178R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Nurr1 polyclonal antibody, unconjugated (bs-0178R) at 1:800 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.