bs-0175R-BF350 [Conjugated Primary Antibody]
TrkB Polyclonal Antibody, AbBy Fluor® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TrkB

Immunogen Range: 401-500/822


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4915

Swiss Prot: Q63604

Source: KLH conjugated synthetic peptide derived from rat TrkB

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

Conjugation: AbBy Fluor® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 90-92


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhu, Xiaoxia, et al. "Xiao Yao San improves depressive-like behavior in rats through modulation of β-arrestin 2 mediated pathways in hippocampus." Evid Based Complement Alternat Med.2014:902516.Read more>>
  • BİR, Ferda, et al. "New immunohistochemical markers in the differential diagnosis of nonsmall cell lung carcinoma." Turkish Journal of Medical Sciences (2016).Read more>>
  • Liu P et al. Harmine Ameliorates Cognitive Impairment by Inhibiting NLRP3 Inflammasome Activation and Enhancing the BDNF/TrkB Signaling Pathway in STZ-Induced Diabetic Rats. Front Pharmacol. 2020 May 1;11:535.Read more>>
  • Zhai X et al. L-lactate preconditioning promotes plasticity-related proteins expression and reduces neurological deficits by potentiating GPR81 signaling in rat traumatic brain injury model. Brain Res. 2020 Nov 1;1746:146945.Read more>>
  • Roberta Passos Palazzo. et al. Early life exposure to hypercaloric diet impairs eating behavior during weaning: The role of BDNF signaling and astrocyte marks. Int J Dev Neurosci. 2020 Nov;80(7):667-678Read more>>
  • Zhang Yan-hui. et al. -Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1 Signaling Pathway in P301S Mice. Front Aging Neurosci. 2020 Aug;12:262Read more>>
  • Akira Tani. et al. Stimulation of functional recovery via neurorepair mechanisms by the traditional Japanese Kampo medicine, Ninjin'yoeito, and physical exercise in a rat ischemic stroke model. J ETHNOPHARMACOL. 2023 Feb;302:115927Read more>>
VALIDATION IMAGES