bs-0170R
[Primary Antibody]
VEGFR1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: VEGFR1
Immunogen Range: 1162-1260/1333
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 54251
Swiss Prot: P53767
Source: KLH conjugated synthetic peptide derived from rat VEGFR1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC, YES1 and PLCG, and may also phosphorylate CBL. Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 147
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Cheng, Gangwei, et al. "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." Cell Biochemistry and Biophysics: 1-6.Read more>>
- Liu, Huayan, et al. "Paeoniflorin attenuates Aβ 1-42-induced inflammation and chemotaxis of microglia in vitro and inhibits NF-κB-and VEGF/Flt-1 signaling pathways." Brain Research (2015).Read more>>
- Liu, Yanhua, et al. "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." International Journal of Molecular Medicine (2017).Read more>>
- Wang et al. Effects of minocycline on apoptosis and angiogenesis-related protein expression in a rat model of intracerebral hemorrhage. (2012) Neural.Regen.Res. 7:595-600Read more>>
- Blanco et al. Phosphatidylserine-selective targeting and anticancer effects of SapC-DOPS nanovesicles on brain tumors. (2014) Oncotarget. 5:7105-18Read more>>
- Ning et al. Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. (2013) PLoS.One. 8:e65217Read more>>
- He J et al. Cell sheets of co‑cultured BMP‑2‑modified bone marrow stromal cells and endothelial progenitor cells accelerate bone regeneration in vitro. Exp Ther Med. 2019 Nov;18(5):3333-3340. Read more>>
- Yuchen Yao. et al. Seasonal expressions of VEGF and its receptors VEGFR1 and VEGFR2 in the prostate of the wild ground squirrels (Spermophilus dauricus). Eur J Histochem. 2021 Mar 24; 65(2): 3219Read more>>
- Zhaocheng Zhang. et al. Inverse and reciprocal regulation of p53/p21 and Bmi-1 modulates vasculogenic differentiation of dental pulp stem cells. Cell Death Dis. 2021 Jun;12(7):1-12Read more>>
- Enoch Odame Anto. et al. Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia. Plos One. 2022 Mar;17(3):e0265717Read more>>
- Qi Rao. et al. Alcohol extracts from Anemone?flaccida Fr. Schmidt treat rheumatoid arthritis via inhibition of synovial hyperplasia and angiogenesis. MOL MED REP. 2023 Apr;27(4):1-12Read more>>
VALIDATION IMAGES
Image kindly submitted by Victor Blanco as part of the Free Sample Program. Frozen mouse brain probed with bs-0170R at 1:100, overnight at 4C.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-VEGFR1/FLT1 Polyclonal Antibody, Unconjugated (bs-0170R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells probed with VEGFR1 Polyclonal Antibody, unconjugated (bs-0170R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (VEGFR1) Polyclonal Antibody, Unconjugated (bs-0170R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Recombinant human VEGFR1 protein, C-His (HEK293)( bs-41611P) probed with beta VEGFR1 Polyclonal Antibody, Unconjugated (bs-0170R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.