bs-0169R
[Primary Antibody]
Caspase-1 P10 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Caspase-1 P10
Immunogen Range: 320-404/404
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 834
Swiss Prot: P29466
Source: KLH conjugated synthetic peptide derived from human Caspase-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: Oct-45
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Others
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Shaolan, et al. "Xenobiotic receptor PXR regulates innate immunity via activation of NLRP3 inflammasome in vascular endothelial cells." Journal of Biological Chemistry (2014): jbc-M114.Read more>>
- Hu, Hanhua, et al. "Drug-induced apoptosis of Echinococcus granulosus protoscoleces." Parasitology research 109.2 (2011): 453-459.Read more>>
- Yao, Xiao, et al. "Estrogen-provided cardiac protection following burn trauma is mediated through a reduction in mitochondria-derived DAMPs." Am J Physiol Heart Circ Physiol 306.6 (2014): H882-H894.Read more>>
- Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103.Read more>>
- Cai, Rentian, et al. "Caspase-1 activity in CD4 T cells is downregulated following antiretroviral therapy for HIV-1 infection." AIDS Research and Human Retroviruses ja (2016).Read more>>
- Liao J et al. Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes.Ecotoxicol Environ Saf. 2019 Jun 15;174:110-119.Read more>>
- Chen Y et al. Curcumin attenuates potassium oxonate-induced hyperuricemia and kidney inflammation in mice. Biomed Pharmacother. 2019 Jul 27;118:109195.Read more>>
- Guan X et al. Estrogen deficiency aggravates apical periodontitis by regulating NLRP3/caspase-1/IL-1β axis. Am J Transl Res. 2020 Feb 15;12(2):660-671.Read more>>
- Shu-Min Yeet al. Silencing of Gasdermin D by siRNA-Loaded PEI-Chol Lipopolymers Potently Relieves Acute Gouty Arthritis through Inhibiting Pyroptosis. Mol Pharm. 2020 Jul 6.Read more>>
- Zhu Hui-Zheng. et al. Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway. Evid-Based Compl Alt. 2021;2021:6673538Read more>>
- Guangrui Chai. et al. NLRP3 Blockade Suppresses Pro-Inflammatory and Pro-Angiogenic Cytokine Secretion in Diabetic Retinopathy. Diabet Metab Synd Ob. 2020; 13: 3047C3058Read more>>
- Wei Yu. et al. Curcumin suppresses doxorubicin-induced cardiomyocyte pyroptosis via a PI3K/Akt/mTOR-dependent manner. Cardiovasc Diagn The. 2020 Aug; 10(4): 752C769Read more>>
- Deng Zhiqin. et al. Interleukin 1 betaCinduced chloride currents are important in osteoarthritis onset: an in vitro study. Acta Bioch Bioph Sin. 2021 MaRead more>>
- Ziyu Meng. et al. Sesamin promotes apoptosis and pyroptosis via autophagy to enhance antitumour effects on murine T-cell lymphoma. J Pharmacol Sci. 2021 Nov;147:26Read more>>
- Lei Wang. et al. Lactobacillus plantarum DP189 Reduces _-SYN Aggravation in MPTP-Induced Parkinsons Disease Mice via Regulating Oxidative Damage, Inflammation, and Gut Microbiota Disorder. J Agr Food Chem. 2022;70(4):11631173Read more>>
- Kathleen Pappritz. et al. Colchicine prevents disease progression in viral myocarditis via modulating the NLRP3 inflammasome in the cardiosplenic axis. 2022 Feb 17Read more>>
- Lin Wang. et al. Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats. Bioengineered. 2022;13(3):7262-7276Read more>>
- Jingya Yuan. et al. Artesunate Protects Pancreatic _-Cells From Streptozotocin-Induced Diabetes via Inhibition of the NLRP3/Caspase-1/GSDMD Pathway. GEN COMP ENDOCR. 2022 Jun;:114068Read more>>
- Wei Liu. et al. Angelica Yinzi alleviates 1-chloro-2,4-dinitrobenzene-induced atopic dermatitis by inhibiting activation of NLRP3 inflammasome and down-regulating the MAPKs/NF-kB signaling pathway. SAUDI PHARM J. 2022 JulRead more>>
- Lei Wang. et al. Cannabidiol Alleviates the Damage to Dopaminergic Neurons in 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine-Induced Parkinsons Disease Mice Via Regulating Neuronal Apoptosis and Neuroinflammation. NEUROSCIENCE. 2022 Aug;498:64Read more>>
- Li, Hua-Jie. et al. Reduction of natural killer cells is associated with poor outcomes in patients with hepatitis B virus-related acute-on-chronic liver failure. HEPATOL INT. 2022 Sep;:1-14Read more>>
- Yangchun Liu. et al. Nicorandil protects against coronary microembolization-induced myocardial injury by suppressing cardiomyocyte pyroptosis via the AMPK/TXNIP/NLRP3 signaling pathway. EUR J PHARMACOL. 2022 Dec;936:175365Read more>>
- Qiangbin Zhu. et al. Expression of histone H1 in rats with traumatic brain injury and the effect of the NLRP3 inflammasome pathway. WORLD NEUROSURG. 2022 DecRead more>>
- Marques-da-Silva, Camila. et al. AIM2 sensors mediate immunity to <i>Plasmodium</i> infection in hepatocytes. P NATL ACAD SCI USA. 2023 Jan;120(2):e221018112Read more>>
- Zhen Xu. et al. Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Zhen Xu. et al. The aqueous extracts of Ageratum conyzoides inhibit inflammation by suppressing NLRP3 inflammasome activation. J ETHNOPHARMACOL. 2023 Jun;309:116353Read more>>
- Congcong Chen. et al. Radix Paeoniae Alba attenuates Radix Bupleuri-induced hepatotoxicity by modulating gut microbiota to alleviate the inhibition of saikosaponins on glutathione synthetase. J PHARM ANAL. 2023 ApRead more>>
- Lihui Ni. et al. The combination of insulin and linezolid ameliorates Staphylococcus aureus pneumonia in individuals with diabetes via the TLR2/MAPKs/NLRP3 pathway. INT J BIOL MACROMOL. 2023 Jul;242:12475Read more>>
- Zijun Zhou. et al. Paeonia lactiflora Pall. Polysaccharide alleviates depression in CUMS mice by inhibiting the NLRP3/ASC/Caspase-1 signaling pathway and affecting the composition of their intestinal flora. J ETHNOPHARMACOL. 2023 Nov;316:116716Read more>>
- Run-Feng Miao. et al. LncRNA CDKN2B-AS1 interacts with LIN28B to exacerbate sepsis-induced acute lung injury by inducing HIF-1/NLRP3-mediated pyroptosis. KAOHSIUNG J MED SCI. 2023 JuRead more>>
- Run-Feng Miao. et al. LncRNA CDKN2B-AS1 interacts with LIN28B to exacerbate sepsis-induced acute lung injury by inducing HIF-1/NLRP3-mediated pyroptosis. KAOHSIUNG J MED SCI. 2023 JuRead more>>
- Li Wei. et al. The CXCL12-CXCR4-NLRP3 axis promotes Schwann cell pyroptosis and sciatic nerve demyelination in rats. CLIN EXP IMMUNOL. 2023 JulRead more>>
- Meei-Ling Sheu. et al. Neuronal Death Caused by HMGB1-Evoked via Inflammasomes from Thrombin-Activated Microglia Cells. INT J MOL SCI. 2023 Jan;24(16):12664Read more>>
- Xueyan Zhang. et al. Role of NLRP3 in the exacerbation of ozone-induced allergic rhinitis. ECOTOX ENVIRON SAFE. 2023 Oct;265:115506Read more>>
- Suli Jiang. et al. CXCL1-CXCR2 axis mediates inflammatory response after sciatic nerve injury by regulating macrophage infiltration. MOL IMMUNOL. 2024 May;169:5Read more>>
- Lin Zhang. et al. Astragaloside IV alleviates renal fibrosis by inhibiting renal tubular epithelial cell pyroptosis induced by urotensin II through regulating the cAMP/PKA signaling pathway. PLOS ONE. 2024 May;19(5):e0304365Read more>>
- Hao Wang. et al. Modulatory Effect of Dihydromyricetin on Chronic Alcoholic Liver Injury Based on the FXR-NLRP3 Signaling Pathway and Serum and Liver Metabolomics. NAT PROD COMMUN. ;()Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-Caspase-1 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 Cells lysates probed with Caspase-1 P10 Polyclonal Antibody, unconjugated (bs-0169R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: U-87MG Cells; 30ug loaded into the lane; Probed with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: U251 Cells; 30ug loaded into the lane; Probed with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin-embedded Human Lung Carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with Caspase-1 P10 Polyclonal Antibody, Unconjugated (bs-0169R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counterstaining.
Hl-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Caspase-1 P10 Polyclonal Antibody(bs-0169R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).