bs-0165R-BF488 [Conjugated Primary Antibody]
EGFR Polyclonal Antibody, AbBy Fluor® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: EGFR

Immunogen Range: 951-1050/1210


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1956

Swiss Prot: P00533

Source: KLH conjugated synthetic peptide derived from human EGFR

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

Conjugation: AbBy Fluor® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 130


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ayers et al. Identification of candidate gonadal sex differentiation genes in the chicken embryo using RNA-seq. (2015) BMC.Genomic. 16:704Read more>>
  • Utomo AW et al. THE ASSOCIATION BETWEEN EXPRESSION OF EPIDERMAL GROWTH FACTOR RECEPTOR WITH PRIMARY TUMOR VOLUME OF NASOPHARYNGEAL CARCINOMA. The International Journal of Nasopharyngeal Carcinoma (IJNPC).2019 1(1). Read more>>
  • Jia M et al. A platform for primary tumor origin identification of circulating tumor cells via antibody cocktail-based in vivo capture and specific aptamer-based multicolor fluorescence imaging strategy. Analytica Chimica Acta.2019 July.Read more>>
  • Kai Liu. et al. Gold nanoparticle enhanced detection of EGFR with a terahertz metamaterial biosensor. Biomed Opt Express. 2021 Mar;12(3):1559-1567Read more>>
  • Wenqian Xie. et al. Seasonal expressions of ER, ER, EGF, EGFR, PI3K and Akt in the scent glands of the muskrats (Ondatra zibethicus). J Steroid Biochem. 2021 Oct;213:105961Read more>>
  • Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-_B pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166Read more>>
  • Xiuxiu Wang. et al. Very-light alcohol consumption suppresses breast tumor progression in a mouse model. Food Funct. 2022 MaRead more>>
  • Rong Li. et al. Integrated Analysis Reveals the Targets and Mechanisms in Immunosuppressive Effect of Mesalazine on Ulcerative Colitis. FRONT NUTR. 2022; 9: 867692Read more>>
  • Zengwei Chen. et al. Quantitative analysis of multiple breast cancer biomarkers using DNA-PAINT. ANAL METHODS-UK. 2022 AugRead more>>
  • ingjie Ge. et al. Integrated network toxicology, molecular docking, and in vivo experiments to elucidate molecular mechanism of aflatoxin B1 hepatotoxicity. ECOTOX ENVIRON SAFE. 2024 Apr;275:116278Read more>>
  • Huang Tian. et al. MAL2 reprograms lipid metabolism in intrahepatic cholangiocarcinoma via EGFR/SREBP-1 pathway based on single-cell RNA sequencing. CELL DEATH DIS. 2024 Jun;15(6):1-18Read more>>
VALIDATION IMAGES