bs-0163R [Primary Antibody]
eNOS Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: eNOS

Immunogen Range: 1105-1202/1203


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4846

Swiss Prot: P29474

Source: KLH conjugated synthetic peptide derived from human NOS-3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 133


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Nour, Sayed, et al. "Intrapulmonary shear stress enhancement: A new therapeutic approach in acute myocardial ischemia." International Journal of Cardiology (2013).Read more>>
  • Li, Hongyan, et al. "Oxidative stress, endothelial dysfunction and inflammatory response in rat heart to NO< sub> 2</sub> inhalation exposure." Chemosphere 82.11 (2011): 1589-1596.Read more>>
  • Ji, Chenyang, et al. "Construction of tissue‐engineered corpus cavernosum with muscle‐derived stem cells and transplantation in vivo." BJU international 107.10 (2011): 1638-1646.Read more>>
  • Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B</sub> receptors and α< sub> 1</sub>-adrenoceptors in spontaneously hypertensive rat arteries." European Journal of Pharmacology (2013).Read more>>
  • Hu, Zhibin, et al. "Comparative analysis of preservation method and intermittent perfusion volume on the expression of endothelial and inflammatory markers by coronal artery and myocardium in porcine donor hearts." ASAIO journal (American Society for Artificial Internal Organs: 1992) (2014).Read more>>
  • Wen, Chen-Ting, Tao He, and Yi-Qiao Xing. "Erythropoietin promotes retinal angiogenesis in a mouse model." Molecular medicine reports 10.6 (2014): 2979-2984.Read more>>
  • Wu, Chun Shan, et al. "Bubble CPAP Support after Discontinuation of Mechanical Ventilation Protects Rat Lungs with Ventilator-Induced Lung Injury." Respiration (2016).Read more>>
  • Prayitnaningsih et al. Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats. (2016) Int.J.Ophthalmol. 9:1568-1577Read more>>
  • Jin et al. Icariin, a phosphodiesterase-5 inhibitor, improves learning and memory in APP/PS1 transgenic mice by stimulation of NO/cGMP signalling. (2014) Int.J.Neuropsychopharmacol. 17:871-81Read more>>
  • Yang et al. In situ eNOS/NO up-regulation-a simple and effective therapeutic strategy for diabetic skin ulcer. (2016) Sci.Rep. 6:30326Read more>>
  • Li B et al. Oxidative stress mediates renal endothelial cell damage in trichloroethylene-sensitized mice. J Toxicol Sci. 2019;44(5):317-326. Read more>>
  • Chen L et al. Compressive force-induced autophagy in periodontal ligament cells downregulatesosteoclastogenesis during tooth movement. J Periodontol. 2019 May 11. Read more>>
  • "Gao Y et al. Ginsenoside Re inhibits vascular neointimal hyperplasia in balloon-injured carotid arteries through activating the eNOS/NO/cGMP pathway in rats Y Gao, CY Gao, P Zhu, SF Xu, YM Luo, J Deng… - Biomedicine & …, 2018Biomed Pharmacother. 2018 Oct;106:1091-1097. "Read more>>
  • Fan Q et al. Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement. Evid Based Complement Alternat Med. 2020 Jun 9;2020:2957094.Read more>>
  • Liang Huang. et al. Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489C3p/Sirt1 axis. J Pharmacol Sci. 2021 AugRead more>>
  • Ali Tu?rul Akin. et al. Therapeutic effects of thymoquinone in doxorubicin-induced hepatotoxicity via oxidative stress, inflammation and apoptosis. 2021 Sep 08Read more>>
  • Gokhan Ozge. et al. Salubrinal Ameliorates Inflammation and Neovascularization via the Caspase 3/Enos Signaling in an Alkaline-Induced Rat Corneal Neovascularization Model. MED LITH. 2023 Feb;59(2):323Read more>>
  • Hongming Sun. et al. Human Cord BloodCEndothelial Progenitor Cells Alleviate Intimal Hyperplasia of Arterial Damage in a Rat Stroke Model. CELL TRANSPLANT. ;()Read more>>
VALIDATION IMAGES

Cultured endothelial cells labeled with Anti-Nos-3/ENOSPolyclonal Antibody (bs-0163R), Unconjugated, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG,FITC conjugated (bs-0295G-FITC) at 1:200 dilution for 40 min.


FACS Analysis of Endothelial Nitric Oxide Synthase (eNOS; NOS3) in Circulating Blood Cells in Mouse using Rabbit Anti-eNOS Polyclonal Antibody (bs-0163R-A647). Image kindly submitted by Nasrul Hoda, PhD, Georgia Regents University