Host: Rabbit
Target Protein: p75 NGF Receptor
Immunogen Range: 301-400/427
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 24596
Swiss Prot: P07174
Source: KLH conjugated synthetic peptide derived from rat NGFR
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 44
Cross Reactive Species:
Human
Mouse
Rat
Others
Predicted Cross Reactive Species:
Dog
Cow
Pig
Horse
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
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- Zhang, Ying, et al. "Exendin-4 promotes proliferation of adipose-derived stem cells through ERK and JNK signaling pathways." In Vitro Cellular & Developmental Biology-Animal (2016): 1-9.Read more>>
- Śmieszek, Agnieszka, et al. "Antioxidant and Anti-Senescence Effect of Metformin on Mouse Olfactory Ensheathing Cells (mOECs) May Be Associated with Increased Brain-Derived Neurotrophic Factor Levels—An Ex Vivo Study." International Journal of Molecular Sciences 18.4 (2017): 872.Read more>>
- Zhang K et al. Multipotent stem cells with neural crest stem cells characteristics exist in bovine adipose tissue. Biochem Biophys Res Commun. 2019 Nov 29.Read more>>
- Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
- Yoshito Mizoguchi. et al. ProBDNF induces sustained elevation of intracellular Ca2+ possibly mediated by TRPM7 channels in rodent microglial cells. 2021 Mar 19Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated (bs-0161R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse brain lysates probed with Anti-p75 NGF Receptor Polyclonal Antibody (bs-0161R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
HepG2 cells were stained with p75 NGF receptor Polyclonal Antibody, Unconjugated(bs-0161R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C
Formalin-fixed and paraffin-embedded rat liver labeled with Anti-NGFR/p75NTR Polyclonal Antibody, Unconjugated(bs-0022R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.
Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NGF Receptor) polyclonal Antibody, Unconjugated (bs-0161R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei
Mouse spleen cells probed with p75 NGF Receptor Polyclonal Antibody, unconjugated (bs-0161R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)