bs-0159R
[Primary Antibody]
Tubulin-alpha Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Tubulin-alpha
Specificity: will recognize many forms of alpha tubulin, Tubulin alpha-1A chain, Tubulin alpha-4A chain etc
Immunogen Range: 375-448/448
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7277
Swiss Prot: P68366
Source: KLH conjugated synthetic peptide derived from human Tubulin-alpha 1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 50
Cross Reactive Species:
Human
Mouse
Rat
Plant
Predicted Cross Reactive Species:
Cow
Sheep
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- FANG, Wan‐Ping, et al. "Differentially Expression of Tua 1, a Tubulin‐encoding Gene, during Flowering of Tea Plant Camellia sinensis (L.) O. Kuntze Using cDNA Amplified Fragment Length Polymorphism Technique." Acta biochimica et biophysica Sinica 38.9 (2006): 653-662.Read more>>
- Wang, Jin, et al. "Clinical and tumor significance of tropomyosin-1 expression levels in renal cell carcinoma." Oncology Reports 33.3 (2015): 1326-1334.Read more>>
- Wang et al. Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model. (2017) Chin.Med.J.(Engl. 130:1026-1032Read more>>
- Sondag et al. Osteoactivin inhibition of osteoclastogenesis is mediated through CD44-ERK signaling. (2016) Exp.Mol.Me. 48:e257Read more>>
- Sondag et al. Osteoactivin inhibition of osteoclastogenesis is mediated through CD44-ERK signaling. (2016) Exp.Mol.Me. 48:e257Read more>>
- Du et al. Silencing of the TPM1 gene induces radioresistance of glioma U251 cells. (2015) Oncol.Re. 33:2807-14Read more>>
- Xiong L et al. Effect of decabrominated diphenyl ether exposure on spatial learning and memory, the expression and phosphorylation of hippocampal glutamate receptor subunits in adult Sprague-Dawley rats.(2018)J Toxicol Sci;43(11):645-657.Read more>>
- Adil Hassan et al. Anti-atherosclerotic effects of Lactobacillus plantarum ATCC 14917 in ApoE ?/? mice through modulation of proinflammatory cytokines and oxidative stress. Appl Microbiol Biotechnol. 2020 Jul;104(14):6337-6350.Read more>>
- Li X et al. MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1. Biosci Rep. 2020 Jun 26;40(6):BSR20191327.Read more>>
- YING-HAO HAN. et al. Peroxiredoxin II Inhibits Alcohol-induced Apoptosis in L02 Hepatocytes Through AKT/-Catenin Signaling Pathway. Anticancer Res. 2020 Aug;40(8):4491-4504Read more>>
- Li He. et al. NETs promote pathogenic cardiac fibrosis and participate in ventricular aneurysm formation after ischemia injury through the facilitation of perivascular fibrosis. Biochem Bioph Res Co. 2021 Dec;583:154Read more>>
- Shu-Ting Miao. et al. Oral administration of octacosanol modulates the gut bacteria and protects the intestinal barrier in ulcerative colitis mice. J FOOD BIOCHEM. 2022 Jun 23Read more>>
- Meiting Li. et al. Acetylation of p62 regulates base excision repair through interaction with APE1. CELL REP. 2022 Jul;40:111116Read more>>
- Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858Read more>>
- Jiashuang Li. et al. Overexpression of Ultrabithorax Changes the Development of Silk Gland and the Expression of Fibroin Genes in Bombyx mori. INT J MOL SCI. 2023 Jan;24(7):667Read more>>
- Yuanyuan Wang. et al. A Metabolomics-Based Study on NMDAR-Mediated Mitochondrial Damage through Calcium Overload and ROS Accumulation in Myocardial Infarction. FRONT BIOSCI-LANDMRK. 2023 Jul;28(7):14Read more>>
- Juan Wang. et al. A novel autosomal dominant ERLIN2 variant activates endoplasmic reticulum stress in a Chinese HSP family. ANN CLIN TRANSL NEUR. 2023 SepRead more>>
VALIDATION IMAGES
L1 rat heart lysates, L2 rat brain lysates probed: Anti-Tubulin-alpha (bs-0159R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 55kDa
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebellum lysates; Lane 4: Mouse Cerebellum lysates probed with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human 293T cell lysates probed with Tubulin-alpha (Loading Control) Polyclonal Antibody, Unconjugated (bs-0159R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human U-2 OS cell lysates; Lane 5: Human A431 cell lysates probed with Tubulin-alpha (Loading Control) Polyclonal Antibody, Unconjugated (bs-0159R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin-alpha Polyclonal Antibody, Unconjugated (bs-0159R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.