bs-0117R
[Primary Antibody]
TGF beta R2 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TGF beta Receptor 2
Immunogen Range: 241-330/567
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7048
Swiss Prot: P37173
Source: KLH conjugated synthetic peptide derived from human TGF beta R2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 62
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Cow
Predicted Cross Reactive Species:
Sheep
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Reduces Bone Formation in Vivo and Osteoblast Differentiation in Vitro." The American Journal of Pathology (2014).Read more>>
- Friedrichs, Jacqueline, et al. "TGF-β1-dependent induction and nuclear translocation of FHL2 promotes keratin expression in pilomatricoma." Virchows Archiv (2014): 1-10.Read more>>
- Hirayama, Hiroki, et al. "Localization of TGF-β and TGF-β receptor in bovine term placentome and expression differences between spontaneous and induced parturition." Placenta (2015).Read more>>
- Li et al. TNF-α enhances the effect of TGF-β on Gli2 expression in the KG-1 leukemic cell line. (2014) Exp.Ther.Me. 8:676-680Read more>>
- Song et al. ERBB3, IGF1R, and TGFBR2 expression correlate with PDGFR expression in glioblastoma and participate in PDGFR inhibitor resistance of glioblastoma cells. (2018) Am.J.Cancer.Res. 8:792-809Read more>>
- Văduva IA et al. SMAD4 and TGFβR2 expression in pancreatic ductal carcinoma. Rom J Morphol Embryol. 2019;60(3):803-809.Read more>>
- Taotao Li. et al. Integrating miRNA and mRNA Profiling to Assess the Potential miRNACmRNA Modules Linked With Testicular Immune Homeostasis in Sheep. Front Vet Sci. 2021; 8: 647153Read more>>
- Longfei Xiao. et al. Dihydrotestosterone through blockade of TGF-/Smad signaling mediates the anti-fibrosis effect under hypoxia in canine Sertoli cells. J Steroid Biochem. 2022 Feb;216:106041Read more>>
- Zihui Wang. et al. Heat stress and hypoxia inhibit the secretion of androgens and induce epithelial-to-mesenchymal transition associated with activated TGF-_/Smad signaling in canine cryptorchidism. REPROD DOMEST ANIM. 2022 Jun 09Read more>>
- aisheng Li. et al. Decrease of circARID1A retards glioblastoma invasion by modulating miR-370-3p/ TGFBR2 pathway. INT J BIOL SCI. 2022; 18(13): 51235135Read more>>
- Xiao, Jiangwei. et al. IRE1 arm of unfolded protein response in muscle-specific TGF- signaling-mediated regulation of muscle cell immunological properties. CELL MOL BIOL LETT. 2023 Dec;28(1):1-24Read more>>
- Xiangrong Chen. et al. ADAM17 Aggravates the Inflammatory Response by Modulating Microglia Polarization Through the TGF-1/Smad Pathway Following Experimental Traumatic Brain Injury. J NEUROTRAUM. 2023 May 22Read more>>
- Liao Zhaohong. et al. Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF- signaling. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-18Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded: rabbit carotid artery labeled with Anti-TGF-beta-R2/TGFBR2 Polyclonal Antibody (bs-0117R), Unconjugated 1:600 followed by conjugation to the secondary antibody and DAB staining
HepG2 cell lysates probed with Rabbit Anti-TGF beta R2 Polyclonal Antibody, Unconjugated (bs-0117R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rhuman placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.