bs-0116R [Primary Antibody]
Estrogen receptor beta Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Estrogen receptor beta

Immunogen Range: 201-300/530


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2100

Swiss Prot: Q92731

Source: KLH conjugated synthetic peptide derived from human ER-beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fang, Ming, Xin-Chi Wu, and Wenlong Huang. "Raloxifene Upregulated Mesangial Cell MMP-2 Activity via ER-?? Through Transcriptional Regulation."Cell Biochemistry and Biophysics (2013): 1-7..Read more>>
  • Wei, Qing, et al. "Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome." Laboratory Investigation 95.7 (2015): 804-816.Read more>>
  • Rárová,et al.The novel brassinosteroid analog BR4848 inhibits angiogenesis in human endothelial cells and induces apoptosis in human cancer cells in vitro.(2018) The Journal of Steroid Biochemistry and Molecular Biology. 178:263-271.Read more>>
  • He,et al.Disposable Morpho menelaus Based Flexible Microfluidic and Electronic Sensor for the Diagnosis of Neurodegenerative Disease.(2018) Advanced Healthcare Materials. 7:.Read more>>
  • Liu R et al. ERβ modulates genistein's cisplatin-enhancing activities in breast cancer MDA-MB-231 cells via P53-independent pathway. Mol Cell Biochem. 2019 Feb 8.Read more>>
  • Liu R et al. ERβ modulates genistein's cisplatin-enhancing activities in breast cancer MDA-MB-231 cells via P53-independent pathway. Mol Cell Biochem. 2019 Feb 8.Read more>>
  • Gao XX et al. Effects of l-arginine on endometrial estrogen receptor α/β and progesterone receptor expression in nutrient-restricted sheep. Theriogenology. 2019 Jul 25;138:137-144.Read more>>
  • Zhong Yuyi. et al. MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells. Front Cell Dev Biol. 2020 Oct;8:1159Read more>>
  • Xiaotian Xu. et al. p-Hydroxybenzoic acid alleviates inflammatory responses and intestinal mucosal damage in DSS-induced colitis by activating ER signaling. J Funct Foods. 2021 Dec;87:104835Read more>>
  • Yan Lyu. et al. 11-Ethoxyviburtinal improves chronic restraint stress-induced anxiety-like behaviors in gender-specific mice via PI3K/Akt and E2/ER signaling pathways. PHYTOTHER RES. 2023 JuRead more>>
  • Hanfei Wang. et al. Mapping the 5-HTergic neural pathways in perimenopausal mice and elucidating the role of oestrogen receptors in 5-HT neurotransmission. HELIYON. 2024 Mar;10:e27976Read more>>
VALIDATION IMAGES

HeLa cells probed with Estrogen receptor beta Polyclonal Antibody, Unconjugated (bs-0116R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-PE) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Formalin-fixed and paraffin embedded rat ovary tissue labeled with Anti-ER beta Polyclonal Antibody, Unconjugated (bs-0116R), followed by conjugation to the secondary antibody and DAB staining


L1 mouse kidney lysates L2 mouse brain lysates probed with Anti ER-beta Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000. Predicted band 58kD. Observed band size: 58kD


Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Mouse Ovary lysates; Lane 4: Mouse Uterus lysates ; Lane 5: Rat Uterus lysates probed with Estrogen receptor beta Polyclonal Antibody, Unconjugated (bs-0116R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Estrogen receptor beta Antibody(bs-0116R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).