bs-0115R
[Primary Antibody]
AKT1/2/3 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: AKT1/2/3
Immunogen Range: 401-479/479
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 207
Swiss Prot: P31749
Source: KLH conjugated synthetic peptide derived from human AKT1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 56
Cross Reactive Species:
Human
Mouse
Rat
Bovine
Pig
Others
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Xu, Hong-Wu, et al. "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." BMC cancer 13.1 (2013): 247.Read more>>
- Li, Xinxin, et al. "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signaling." Bioscience Reports (2014).Read more>>
- Xu, C., et al. "Proteomics Analysis of Hepatocyte Proliferation Regulated by FGF, PDGF, Insulin, Oncostatin M and Interleukin 2 Signaling Pathways during Rat Liver Regeneration." J Proteomics Computational Biol 1.1 (2014): 8.Read more>>
- Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
- Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
- Tang et al. The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury. (2013) Evid.Based.Complement.Alternat.Me. 2013:820695Read more>>
- Zhang et al. MicroRNA-26b Modulates the NF-κB Pathway in Alveolar Macrophages by Regulating PTEN. (2015) J.Immuno. 195:5404-14Read more>>
- Kumar et al. Simultaneous targeting of 5-LOX-COX and ODC block NNK-induced lung adenoma progression to adenocarcinoma in A/J mice. (2016) Am.J.Cancer.Re. 6:894-909Read more>>
- Liang et al. Histone demethylase RBP2 induced by Helicobactor Pylori CagA participates in the malignant transformation of gastric epithelial cells. (2014) Oncotarge. 5:5798-807Read more>>
- Chen et al. Timosaponin A-III reverses multi-drug resistance in human chronic myelogenous leukemia K562/ADM cells via downregulation of MDR1 and MRP1 expression by inhibiting PI3K/Akt signaling pathway. (2016) Int.J.Oncol. 48:2063-70Read more>>
- Erbas et al. Oxytocin alleviates cisplatin-induced renal damage in rats. (2014) Iran.J.Basic.Med.Sci. 17:747-52Read more>>
- Liu Y et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. Int. J. Mol. Med. Sep 18Read more>>
- Chen Y et al. Antitumor effects of the silencing of programmed cell death ligand 1 in colorectal cancer via immunoregulation. (2018) Oncol.Rep. Read more>>
- Liu et al. Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala. (2018) Mol.Pain. 14:1744806918805902Read more>>
- Liu et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. (2018) Int.J.Mol.Med. 42:3037-3046Read more>>
- Zheng R et al. Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears. Cell Tissue Res. 2019 Apr 24.Read more>>
- Li B et al. Resistin up-regulates LPL expression through the PPARγ-dependent PI3K/AKT signaling pathway impacting lipid accumulation in RAW264. 7 macrophages.Cytokine. 2019 Jul;119:168-174.Read more>>
- Li X et al. NDRG1 negatively regulates proliferation and Milk bio-synthesis of bovine epithelial cells via the mTOR signaling pathway.Res Vet Sci. 2019 Jun;124:158-165. Read more>>
- Tang Q et al. Aqueous extract from You-Gui-Yin ameliorates cognitive impairment of chronic renal failure micethrough targeting hippocampal CaMKIIα/CREB/BDNF and EPO/EPOR pathways. J Ethnopharmacol. 2019 Jul 15;239:111925. Read more>>
- Zhang H et al. Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signalingpathway. Life Sci. 2019 Jul 15;229:210-218. Read more>>
- Wang Z et al. Light-activatable dual prodrug polymer nanoparticle for precise synergistic chemotherapy guidedby drug-mediated computed tomography imaging. Acta Biomater. 2019 May 22. Read more>>
- Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
- Wang Y et al. H2S mediates apoptosis in response to inflammation through PI3K/Akt/NFκB signaling pathway. Biotechnol Lett. 2019 Dec 23. Read more>>
- Wang J et al. Calcium relieves fluoride-induced bone damage through the PI3K/AKT pathway. Food Funct. 2019 Dec 24. Read more>>
- Liu F et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019 Oct 18;120:109533. Read more>>
- Kamohara A et al. IgG immune complexes with Staphylococcus aureus protein A enhance osteoclast differentiation and bone resorption by stimulating Fc receptors and TLR2. Int Immunol. 2019 Nov 12. pii: dxz063. Read more>>
- Zhang Y et al. Overexpressed CES2 has prognostic value in CRC and knockdown CES2 reverses L-OHP-resistance in CRC cells by inhibition of the PI3K signaling pathway. Exp Cell Res. 2020 Jan 22:111856. Read more>>
- Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. MedChemComm. 2018.Read more>>
- Yuan L et al. Long non-coding RNA H19 protects H9c2 cells against hypoxia‑induced injury by activating the PI3K/AKT and ERK/p38 pathways. Molecular Medicine Reports, (2020). 21, 1709-1716. Read more>>
- Kim K et al. Enterococcus faecium L-15 Extract Enhances the Self-Renewal and Proliferation of Mouse Skin-Derived Precursor Cells. Probiotics Antimicrob Proteins. 2020 Mar 11.Read more>>
- Liu B et al. MicroRNA‐379 mediates pigmentation, migration, and proliferation of melanocytes by targeting the insulin‐like growth factor 1 receptor. Exp Dermatol. 2020 May;29(5):467-476.Read more>>
- Chen Y et al. Epothilone B prevents lipopolysaccharide-induced inflammatory osteolysis through suppressing osteoclastogenesis via STAT3 signaling pathway. Aging (Albany NY). 2020 Jun 11;12(12):11698-11716.Read more>>
- Li X et al. MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1. Biosci Rep. 2020 Jun 26;40(6):BSR20191327.Read more>>
- Lijing Wanget al. 1-(4-((5-chloro-4-((2-(isopropylsulfonyl)phenyl)amino)pyrimidin-2-yl)amino)-3-methoxyphenyl)-3-(2-(dimethylamino)ethyl)imidazolidin-2-one (ZX-42), a novel ALK inhibitor, induces apoptosis and protective autophagy in H2228 cells. J Pharm Pharmacol. 2020 Oct;72(10):1370-1382.Read more>>
- Ma Z et al. bFGF promotes neurological recovery from neonatal hypoxicCischemic encephalopathy by IL\1 signaling pathway\mediated axon regeneration. Brain Behav. 2020 Aug;10(8):e01696.Read more>>
- Muhong Guoet al. ROR2 knockdown suppresses breast cancer growth through PI3K/ATK signaling. Aging (Albany NY). 2020 Jul 2;12(13):13115-13127.Read more>>
- Lei Yuet al. TMF, a natural dihydroflavonoid isolated from Scutellaria javanica Jungh, stimulates anticancer activity of s180 cancer-bearing mice, induces apoptosis, inhibits invasion and migration on HepG-2?cells. J Ethnopharmacol. 2020 Dec 5;263:113072.Read more>>
- Lijun Wu. et al. PD-1/PD-L1 enhanced cisplatin resistance in gastric cancer through PI3K/AKT mediated P-gp expression. Int Immunopharmacol. 2021 May;94:107443Read more>>
- Yu Wang. et al. Environmentally relevant concentration of sulfamethoxazole-induced oxidative stress-cascaded damages in the intestine of grass carp and the therapeutic application of exogenous lycopene. Environ Pollut. 2021 Apr;274:116597Read more>>
- Hirohito Hirata. et al. PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking. Faseb J. 2021 Feb;35(2):e21281Read more>>
- Ryeo-Eun Go. et al. Fenhexamid induces cancer growth and survival via estrogen receptor-dependent and PI3K-dependent pathways in breast cancer models. Food Chem Toxicol. 2021 Mar;149:112Read more>>
- Yuling Tong. et al. Zuojin Pill ameliorates chronic atrophic gastritis induced by MNNG through TGF-1/PI3K/Akt axis. J Ethnopharmacol. 2021 May;271:113893Read more>>
- Han Chen. et al. ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-B signaling pathway. Bmc Cancer. 2020 Dec;20(1):1-1Read more>>
- Xinjia Han. et al. Nicotine Alleviates Cortical Neuronal Injury by Suppressing Neuroinflammation and Upregulating Neuronal PI3K-AKT Signaling in an Eclampsia-Like Seizure Model. Neurotox Res. 2020 Oct;38(3):665-681Read more>>
- Xue Zhou. et al. Risk and mechanism of glucose metabolism disorder in the offspring conceived by female fertility maintenance technology. Cryobiology. 2020 Oct;96:68Read more>>
- Lv Qing. et al. Allicin suppresses growth and metastasis of gastric carcinoma: the key role of microRNA-383-5p-mediated inhibition of ERBB4 signaling. Biosci Biotech Bioch. 2020 Oct;84(10):1997-2004Read more>>
- Jin Dai. et al. NK6 Homeobox 2 Regulated Gastrokin-2 Suppresses Gastric Cancer Cell Proliferation and Invasion via Akt Signaling Pathway. Cell Biochem Biophys. 2021 Mar;79(1):123-131Read more>>
- Myoung Su Choi. et al. Activation of the Complement System on Human Endothelial Cells by Urban Particulate Matter Triggers Inflammation-Related Protein Production. Int J Mol Sci. 2021 Jan;22(7):3336Read more>>
- Yi Chen. et al. The essential oil from the raw and vinegar processed Rhizoma Curcumae ameliorate CCl4-incuded liver fibrosis: integrating network pharmacology and molecular mechanism evaluation. 2021 Mar 17Read more>>
- Dahan Yang. et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus. Front Vet Sci. 2021; 8: 666512Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Placenta lysates；Lane 2: Mouse Lung lysates probed with AKT1/2/3 Polyclonal Antibody, unconjugated (bs-0115R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (AKT1) polyclonal Antibody, Unconjugated (bs-0115R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.