bs-0109R [Primary Antibody]
Leptin Receptor Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Leptin receptor (Isoform B)

Specificity: This antibody will recognize the Isoform B variant only

Immunogen Range: 901-1000/1165


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3953

Swiss Prot: P48357

Source: KLH conjugated synthetic peptide derived from human Leptin receptor:901-1000/1165

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 126


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Goto et al. Leptin deficiency down-regulates IL-23 production in glomerular podocytes resulting in an attenuated immune response in nephrotoxic serum nephritis. (2016) Int.Immuno. 28:197-208Read more>>
  • Qiu JT et al. Micro RNA‐7 inhibits melatonin synthesis by acting as a linking molecule between leptin and norepinephrine signaling pathways in pig pineal gland. Journal of Pineal Research,2019 e12552. Read more>>
  • Annamria Schaffer. et al. The ontogenies of endometrial and myometrial leptin and adiponectin receptors in pregnant rats: Their putative impact on uterine contractility. Life Sci. 2022 May;297:120465Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat colon tissue labeled with Rabbit Anti-Leptin receptor(long) Polyclonal Antibody (bs-0109R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Leptin Receptor Polyclonal Antibody(bs-0109R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).