Host: Rabbit
Target Protein: PKM2
Immunogen Range: 255-350/531
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5315, 5313
Swiss Prot: P14618, P30613
Source: KLH conjugated synthetic peptide derived from mouse Pyruvate kinase PKM
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 57
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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- Li, Hongyan, et al. "Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes." Toxicology Letters (2014).Read more>>
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- Jiang, Bin. et al. Role of Proximal Intestinal Glucose Sensing and Metabolism in the Blood Glucose Control in Type 2 Diabetic Rats After Duodenal Jejunal Bypass Surgery. Obes Surg. 2022 Jan;:1-11Read more>>
- Changhao Jia. et al. Apigenin sensitizes radiotherapy of mouse subcutaneous glioma through attenuations of cell stemness and DNA damage repair by inhibiting NF-_B/HIF-1_-mediated glycolysis. J NUTR BIOCHEM. 2022 May;:109038Read more>>
- Sun, Xin-Gang. et al. Aerobic Glycolysis Induced by mTOR/HIF-1 Promotes Early Brain Injury After Subarachnoid Hemorrhage via Activating M1 Microglia. TRANSL STROKE RES. 2022 Nov;:1-15Read more>>
- Huang, Hui. et al. Osthole increases the radiosensitivity of hepatoma cells by inhibiting GSK-3/AMPK/mTOR pathway-controlled glycolysis. N-S ARCH PHARMACOL. 2022 Nov;:1-1Read more>>
- Shuhong Kang. et al. Hsa_circ_0008133 contributes to lung cancer progression by promoting glycolysis metabolism through the miR-760/MEX3A axis. ENVIRON TOXICOL. 2024 FebRead more>>
- Jun Ma. et al. The Protective Effect of Quercetin against the Cytotoxicity Induced by Fumonisin B1 in Sertoli Cells. INT J MOL SCI. 2024 Jan;25(16):8764Read more>>
- Weipeng Zhang. et al. Cross-generational effects of dietary sea buckthorn on non-alcoholic fatty liver disease in offspring of obese female mice. J FUNCT FOODS. 2024 Oct;121:106398Read more>>
Mouse heart lysates probed with PKM2 Polyclonal Antibody, unconjugated (bs-0101R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Lane 1: Rat Testis lysates; Lane 2: Rat Prostate lysates; Lane 3: Rat Kidney lysates probed with PKM2 Polyclonal Antibody, Unconjugated (bs-0101R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PKM2 Polyclonal Antibody(bs-0101R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).