Host: Rabbit
Target Protein: CA125
Immunogen Range: 250-350/2008
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 94025
Swiss Prot: Q8WXI7
Source: KLH conjugated synthetic peptide derived from human CA125
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 200
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Xie, Chao, Chaoqing Dong, and Jicun Ren. "Study on homogeneous competitive immune reaction by fluorescence correlation spectroscopy: Using synthetic peptide as antigen." Talanta 79.3 (2009): 971-974.Read more>>
- Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415.Read more>>
- Guo, Yuna, et al. "R-ketorolac Targets Cdc42 and Rac1 and Alters Ovarian Cancer Cell Behaviors Critical for Invasion and Metastasis." Molecular Cancer Therapeutics (2015): molcanther-0419.Read more>>
- Zong, Chen, et al. "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." Biosensors and Bioelectronics 43 (2013): 372-378.Read more>>
- Liu et al. Increased autophagy in EOC re-ascites cells can inhibit cell death and promote drug resistance. (2018) Cell.Death.Dis. 9:419Read more>>
Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MUC16 Polyclonal Antibody, Unconjugated (bs-0091R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.