Host: Rabbit
Target Protein: VIP
Immunogen Range: 125-152/170
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7432
Swiss Prot: P01282
Source: KLH conjugated synthetic peptide derived from human VIP
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 3.3/18
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Wieck, Minna M., et al. "Human and murine tissue-engineered colon exhibit diverse neuronal subtypes and can be populated by enteric nervous system progenitor cells when donor colon is aganglionic." Tissue Engineering A (2015).Read more>>
- Liang et al. Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome. (2016) Gut.Liver. 10:382-90Read more>>
- Wieck et al. Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic. (2016) Tissue.Eng.Part.A. 22:53-64Read more>>
- Liu Dan. et al. RSV Promotes Epithelial Neuroendocrine Phenotype Differentiation through NODAL Signaling Pathway. Biomed Res Int. 2021;2021:9956078Read more>>
- Zhiqiang Zhang. et al. Vasoactive Intestinal Peptide (VIP) Protects Nile Tilapia (Oreochromis niloticus) against Streptococcus agalatiae Infection. INT J MOL SCI. 2022 Jan;23(23):14895Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Anti-VIP Polyclonal Antibody, Unconjugated (bs-0077R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti- VIP Antibody, conjugated (bs-0077R-AF555) at 1:200 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti- VIP Antibody, conjugated (bs-0077R-AF555) at 1:200 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.