Host: Rabbit
Target Protein: NGF beta
Immunogen Range: 151-220/241
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4803
Swiss Prot: P01138
Source: KLH conjugated synthetic peptide derived from human NGF beta
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 13/32
Cross Reactive Species:
Human
Mouse
Rat
Dog
Predicted Cross Reactive Species:
Cow
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
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- Shu et al. Connexin 43 is involved in the sympathetic atrial fibrillation in canine and canine atrial myocytes. (2017) Anatol.J.Cardiol. 18:3-9Read more>>
- Fu XM et al. The Combination of Adipose-derived Schwann-like Cells and Acellular Nerve Allografts PromotesSciatic Nerve Regeneration and Repair through the JAK2/STAT3 Signaling Pathway in Rats. Neuroscience. 2019 Nov 1;422:134-145. Read more>>
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- Zhou, Zhen. et al. Metabolism regulator adiponectin prevents cardiac remodeling and ventricular arrhythmias via sympathetic modulation in a myocardial infarction model. BASIC RES CARDIOL. 2022 Dec;117(1):1-17Read more>>
- Zhijie Shen. et al. Renal denervation improves mitochondrial oxidative stress and cardiac hypertrophy through inactivating SP1/BACH1-PACS2 signaling. INT IMMUNOPHARMACOL. 2024 Nov;141:112778Read more>>
Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-NGF Polyclonal Antibody, Unconjugated (bs-0067R) at 1:300 overnight at 4°C, followed by a conjugated secondary and DAB staining\n
Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-NGF-beta Polyclonal Antibody (bs-0067R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Brain tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Human U87MG cell Lysates; Lane 4: Human SH-SY5Y cell Lysates; Lane 5: Human U2 OS cell Lysates probed with NGFB Polyclonal Antibody, Unconjugated (bs-0067R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.