DATASHEET
Host:
Rabbit
Target Protein:
TGF alpha
Immunogen Range:
40-89/160
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
7039
Swiss Prot:
P01135
Source:
KLH conjugated synthetic peptide derived from human TGF-alpha
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
PRODUCT SPECIFIC PUBLICATIONS
- Sun et al. The correlation between microRNA490-3p and TGFα in endometrial carcinoma tumorigenesis and progression. (2016) Oncotarge. 7:9236-49Read more>>
VALIDATION IMAGES
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Colon tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Rat Kidney tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human THP-1 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human U87MG cell lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Kidney tissue lysates; Lane 4: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.