bs-0066R [Primary Antibody]
TGF alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TGF alpha

Immunogen Range: 40-89/160


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7039

Swiss Prot: P01135

Source: KLH conjugated synthetic peptide derived from human TGF-alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 17


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun et al. The correlation between microRNA490-3p and TGFα in endometrial carcinoma tumorigenesis and progression. (2016) Oncotarge. 7:9236-49Read more>>
VALIDATION IMAGES

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Colon tissue lysates; Lane 3: Mouse Spleen tissue lysates; Lane 4: Rat Kidney tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Rat Spleen tissue lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human THP-1 cell lysates; Lane 9: Human A431 cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human U87MG cell lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Kidney tissue lysates; Lane 4: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Liver tissue lysates probed with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.