bs-0064R [Primary Antibody]
Neurokinin 1 Receptor Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Neurokinin 1 Receptor

Immunogen Range: 2-50/407


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6869

Swiss Prot: P25103

Source: KLH conjugated synthetic peptide derived from human Neurokinin 1 Receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.

Size: 100ul

Concentration: 1ug/ul

Applications: WB()
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 45


Cross Reactive Species: Human
Rat
Others

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig
Horse
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • 任绪莹, et al. "小半夏汤对水貂呕吐模型作用机制的研究." Acta Neuropharmacologica 1.3 (2011).Read more>>
  • 杨玉玲, et al. "姜辣素辅助昂丹司琼对顺铂所致大鼠异嗜行为的抑制作用." 世界华人消化杂志 19.10 (2011): 1034-1039.Read more>>
  • Zhang et al. Effects of Neuropeptide Substance P on Proliferation and β-Cell Differentiation of Adult Pancreatic Ductal Cells. (2018) Front.Neurosci. 12:807Read more>>
  • Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25. Read more>>
  • Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306Read more>>
  • Jimei Zhang. et al. Stigmasterol alleviates allergic airway inflammation and airway hyperresponsiveness in asthma mice through inhibiting substance-P receptor. PHARM BIOL. 2023;61(1):449-458Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SPR Polyclonal Antibody, Unconjugated (bs-0064R) 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Anti Substance P Receptor/NK1R Polyclonal Antibody, Unconjugated (bs-0064R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C.


HeLa lysates probed with Neurokinin 1 Receptor Polyclonal Antibody, unconjugated (bs-0064R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


U251 lysates probed with Neurokinin 1 Receptor Polyclonal Antibody, unconjugated (bs-0064R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HeLa cell lysates probed with Neurokinin 1 Receptor Polyclonal Antibody, Unconjugated (bs-0064R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.