bs-0052R [Primary Antibody]
CASP8 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CASP8

Immunogen Range: 411-482/482


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12370

Swiss Prot: Q9JHX4

Source: KLH conjugated synthetic peptide derived from rat CASP8 subunit p10

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP1. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: Dec-55


Cross Reactive Species: Human
Mouse
Rat
Pig
Sheep
Monkey

Predicted Cross Reactive Species: Dog
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Gang, et al. "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." Apoptosis (2013): 1-15.Read more>>
  • Wu, Chun-Yan, et al. "PCSK9 siRNA Inhibits HUVEC Apoptosis Induced by ox-LDL via Bcl/Bax–Caspase9–Caspase3 Pathway." Molecular and Cellular Biochemistry 359.1-2 (2012): 347-358.Read more>>
  • Fang C, Zhang J, Qi D, Fan X, Luo J, et al. (2014) Evodiamine Induces G2/M Arrest and Apoptosis via Mitochondrial and Endoplasmic Reticulum Pathways in H446 and H1688 Human Small-Cell Lung Cancer Cells. PLoS ONE 9(12): e115204. Read more>>
  • Tulsulkar, Jatin, et al. "Ginkgo biloba Extract Prevents Female Mice from Ischemic Brain Damage and the Mechanism Is Independent of the HO1/Wnt Pathway." Translational Stroke Research (2015): 1-12.Read more>>
  • Gao, Hui, et al. "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer."American Journal of Translational Research 8.2 (2016): 1115-1132.Read more>>
  • Akinrinde, A. S., et al. "Alterations in blood pressure, antioxidant status and caspase 8 expression in cobalt chloride-induced cardio-renal dysfunction are reversed by Ocimum gratissimum and gallic acid in Wistar rats." Journal of Trace Elements in Medicine and Biology 36 (2016): 27-37.Read more>>
  • Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103.Read more>>
  • Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).Read more>>
  • Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
  • Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
  • Pang and Fan Cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial cells in vitro. (2016) Int.J.Ophthalmo. 9:497-504Read more>>
  • Yuan et al. Cytotoxicity of pilocarpine to human corneal stromal cells and its underlying cytotoxic mechanisms. (2016) Int.J.Ophthalmo. 9:505-11Read more>>
  • Peng et al. Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells I n -v itro. (2015) Iran.J.Pharm.Re. 14:251-61Read more>>
  • Wang Q et al. Cdc20 and molecular chaperone CCT2 and CCT5 are required for the Muscovy duck reovirus p10. 8-induced cell cycle arrest and apoptosis. Vet Microbiol. 2019 Jun 28;235:151-163.Read more>>
  • Wang X et al. Dibutyl phthalate-mediated oxidative stress induces splenic injury in mice and the attenuating effects of vitamin E and curcumin. Food Chem Toxicol. 2019 Nov 8:110955. Read more>>
  • "Kang K et al. Dexmedetomidine protects against lipopolysaccharide-induced sepsis-associated acute kidney injury via an α7 nAChR-dependent pathway.Biomed Pharmacother. 2018 Oct;106:210-216. "Read more>>
  • Liu F et al. Bioinformatic and experimental findings to indicate anti-cancer targets and mechanisms of calycosin against nasopharyngeal carcinoma. Research Square;12 February 2020.Read more>>
  • Shuli Shao. et al. Toosendanin induces apoptosis of MKN?45 human gastric cancer cells partly through miR?23a?3p?mediated downregulation of BCL2. Mol Med Rep. 2020 Sep;22(3):1793-1802Read more>>
  • Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379Read more>>
  • Rui Feng. et al. Progesterone regulates inflammation and receptivity of cells via the NF-_B and LIF/STAT3 pathways. THERIOGENOLOGY. Theriogenology. 2022 Jul;186:5Read more>>
  • Anvita Gupta. et al. Androgen Receptor Activation Induces Senescence in Thyroid Cancer Cells. CANCERS. 2023 Jan;15(8):2198Read more>>
  • Xin Cao. et al. Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 AugRead more>>
  • Miaomiao Wang. et al. A Mutation Losing an RBP-Binding Site in the LncRNA NORSF Transcript Influences Granulosa Cell Apoptosis and Sow Fertility. ADV SCI. 2024 Aug;:2404747Read more>>
VALIDATION IMAGES

Image provided by the Independent Validation Program (badge number 29759). Lane 1: HeLa cell extract, Lane 2: c6/36 mosquito cell extract (non-reactive\nspecies) probed with Rabbit Anti-Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.


Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Caspase 8 Polyclonal Antibody (bs-0052R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 12kD. Observed band size:48kD.\n


Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase 8) polyclonal Antibody, Unconjugated (bs-0052R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Lane 1: Human Jurkat cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U87MG cell lysates probed with caspase-8 subunit p18 Polyclonal Antibody, Unconjugated (bs-0052R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.