Host: Rabbit
Target Protein: MLANA/MART1
Immunogen Range: 51-118/118
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2315
Swiss Prot: Q16655
Source: KLH conjugated synthetic peptide derived from human MLANA/MART1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 13
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
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Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLANA/MART1 Polyclonal Antibody (bs-0051R) at 1:400 overnight at 4°C, followed by SP Kit (Rabbit) according to instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MLANA/MART1 Polyclonal Antibody, Unconjugated (bs-0051R) at 1:200 overnight at 4°C, DAB staining.