bs-0050R-Cy3 [Conjugated Primary Antibody]
Caspase-9 Polyclonal Antibody, Cy3 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Caspase-9

Immunogen Range: 271-314/416


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 842

Swiss Prot: P55211

Source: KLH conjugated synthetic peptide derived from human Caspase-9 subunit p35

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Conjugation: Cy3

Excitation/ Emission: 512,550nm/570,615nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 35/50


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Hui, et al. "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer."American Journal of Translational Research 8.2 (2016): 1115-1132.Read more>>
  • Yin, Tao, et al. "Bmi1 inhibition enhances the sensitivity of pancreatic cancer cells to gemcitabine." Oncotarget (2016).Read more>>
  • Shan, Ming, and Ting-Jun Fan. "Cytotoxicity of carteolol to human corneal epithelial cells by inducing apoptosis via triggering the Bcl-2 family protein-mediated mitochondrial pro-apoptotic pathway." Toxicology in Vitro (2016).Read more>>
  • Pang and Fan Cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial cells in vitro. (2016) Int.J.Ophthalmo. 9:497-504Read more>>
  • Shen S et al. Transcription factor early growth response-1 plays an oncogenic role in salivary gland pleomorphic adenoma. Biotechnol Lett. 2019 Nov 30.Read more>>
  • Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27. Read more>>
  • Zhang Wei. et al. C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo. Bioscience Rep. 2021 Jan;41(1):BSR20201541Read more>>
  • Fang Cao. et al. Ginkgo biloba L. extract prevents steroid-induced necrosis of the femoral head by rescuing apoptosis and dysfunction in vascular endothelial cells via the PI3K/AKT/eNOS pathway. J ETHNOPHARMACOL. 2022 Jun;:115476Read more>>
  • Masaya Kusunose. et al. Preoperative Increases in T2-Weighted Fat-Suppressed MRI Signal Intensities Associated with Advanced Tissue Degeneration and Mitochondrial Dysfunction in Rotator Cuff Tears. ARTHROSCOPY. 2024 AugRead more>>
VALIDATION IMAGES