bs-0046R [Primary Antibody]
AD7c-NTP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Neuronal thread protein AD7c-NTP

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Neuronal thread protein AD7c-NTP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene is unusual in that its coding sequence is derived almost entirely from a cluster of different Alu repeat sequences. However, the mRNA and the encoded protein have been shown to be expressed in neurons, and overexpressed in brains with Alzheimer's disease. In vitro studies also demonstrated that abnormal expression of this gene promoted neuritic sprouting and cell death, associated with dementia in Alzheimer's disease.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human brain labeled with Anti AD7c-NTP Polyclonal Antibody, Unconjugated (bs-0046R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebellum tissue lysates probed with Neuronal thread protein AD7c-NTP Polyclonal Antibody, Unconjugated (bs-0046R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ( AD7c-NTP) Polyclonal Antibody, Unconjugated (bs-0046R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.