Host: Rabbit
Target Protein: ChAT
Immunogen Range: 101-200/748
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1103
Swiss Prot: P28329
Source: KLH conjugated synthetic peptide derived from human ChAT
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 82
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
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- Wei Lu. et al. Effects of targeted muscle reinnervation on spinal cord motor neurons in rats following tibial nerve transection. Neural Regen Res. 2022 Jan;17(8):1827Read more>>
- Xiaokereti, Jiasuoer. et al. Renal denervation alleviates chronic obstructive sleep apnea-induced atrial fibrillation via inhibition of atrial fibrosis and sympathetic hyperactivity. SLEEP BREATH. 2023 Feb;:1-14Read more>>
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SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ChAT Polyclonal Antibody(bs-0042R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).