bs-0033R-PE
[Conjugated Primary Antibody]
P53 protein(wt-p53) Polyclonal Antibody, PE Conjugated
DATASHEET
Host: Rabbit
Target Protein: P53 proteinwt-p53
Immunogen Range: 251-310/393
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7157
Swiss Prot: P04637
Source: KLH conjugated synthetic peptide derived from human P53
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Conjugation: PE
Excitation/ Emission: 496,564nm/578nm
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
Predicted Molecular Weight: 43
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464.Read more>>
- Wang, Chao, et al. "Hypoxia inhibits myogenic differentiation through p53-dependent induction of Bhlhe40." Journal of Biological Chemistry (2015): jbc-M115.Read more>>
- Hong, Haizheng, et al. "Developmental toxicity of three hexabromocyclododecane diastereoisomers in embryos of the marine medaka Oryzias melastigma." Marine pollution bulletin (2015).Read more>>
- Celik‐Ozenci, C., et al. "Inhibition of poly (ADP‐ribose) polymerase may have preventive potential for varicocoele‐associated testicular damage in rats." Andrology (2016).Read more>>
- Sukohar, Asep, and Mohammad Kanedi. "Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and P53, in T47D Breast Cancer Cells." Biomedical and Pharmacology Journal 10.2 (2017): 641-649.Read more>>
- Uchiumi et al. Characterization of the 5'-flanking region of the human TP53 gene and its response to the natural compound, Resveratrol. (2016) J.Bioche. 159:437-47Read more>>
- Celik-Ozenci et al. Inhibition of poly(ADP-ribose) polymerase may have preventive potential for varicocoele-associated testicular damage in rats. (2017) Andrology. 5:362-369Read more>>
- Liu F et al. Bioinformatic and experimental findings to indicate anti-cancer targets and mechanisms of calycosin against nasopharyngeal carcinoma. Research Square;12 February 2020.Read more>>
- Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
- Tan J et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020 Jun;126:110096.Read more>>
- Liu F et al. Anticancer targets and mechanisms of calycosin to treat nasopharyngeal carcinoma. Biofactors. 2020 Jul;46(4):675-684.Read more>>
- Wenlan Yu. et al. Chronic tribasic copper chloride exposure induces rat liver damage by disrupting the mitophagy and apoptosis pathways. Ecotox Environ Safe. 2021 Apr;212:111968Read more>>
- Yunping Lu. et al. Peroxiredoxin1 Knockdown Inhibits Oral Carcinogenesis via Inducing Cell Senescence Dependent on Mitophagy. Oncotargets Ther. 2021; 14: 239C251Read more>>
- Ling-Chu Hung. et al. The Monoclonal Antibody Recognized the Open Reading Frame Protein in Porcine Circovirus Type 2-Infected Peripheral Blood Mononuclear Cells. Viruses-Basel. 2020 Sep;12(9):961Read more>>
- Yajing Wang. et al. Long-term exposure to the fluoride blocks the development of chondrocytes in the ducks: The molecular mechanism of fluoride regulating autophagy and apoptosis. Ecotox Environ Safe. 2021 Jul;217:112225Read more>>
- Nisreen H. Shehatta. et al. Baicalin; a promising chemopreventive agent, enhances the antitumor effect of 5-FU against breast cancer and inhibits tumor growth and angiogenesis in Ehrlich solid tumor. Biomed Pharmacother. 2022 Feb;146:112599Read more>>
- Marwa A Mahmoud. et al. Rosmarinic acid suppresses inflammation, angiogenesis, and improves paclitaxel induced apoptosis in a breast cancer model via NF3 B-p53-caspase-3 pathways modulation. J Appl Biomed. 2021 Dec;19(4):202-209Read more>>
- Jun Li. et al. In silico studies reveal the anti-osteosarcoma targets and action mechanisms of resveratrol. PROCESS BIOCHEM. Process Biochem. 2022 Jun;117:191Read more>>
- Rui Feng. et al. Progesterone regulates inflammation and receptivity of cells via the NF-_B and LIF/STAT3 pathways. THERIOGENOLOGY. Theriogenology. 2022 Jul;186:5Read more>>
- Hu, Zhuoying. et al. Effects of Long-Term Exposure to Copper on Mitochondria-Mediated Apoptosis in Pig Liver. BIOL TRACE ELEM RES. 2022 Jun;:1-14Read more>>
- Ruoyang Lin. et al. Inhibitory Effects of Rabdosia rubescens in Esophageal Squamous Cell Carcinoma: Network Pharmacology and Experimental Validation. EVID-BASED COMPL ALT. 2022 Nov 10;2022:2696347Read more>>
- Moyu Wang. et al. TRIM25 participates in the fibrous tissue hyperplasia induced by ALV-J infection in chickens by targeting 14-3-3 protein. RES VET SCI. 2023 Feb;155:126Read more>>
- Liang-Hsuan Chien. et al. Evaluation of lung protection of Sanghuangporus sanghuang through TLR4/NF-B/MAPK, keap1/Nrf2/HO-1, CaMKK/AMPK/Sirt1, and TGF-/SMAD3 signaling pathways mediating apoptosis and autophagy. BIOMED PHARMACOTHER. 2023 Sep;165:11508Read more>>
- ohan Chen. et al. Inhalation of ammonia promotes apoptosis and induces autophagy in hepatocytes via Bax/BCl-2 and m-TOR/ATG5/LC-3bII axes. SCI TOTAL ENVIRON. 2024 Feb;912:169036Read more>>
- Guanglin Lu. et al. Nanoplastics trigger the aging and inflammation of porcine kidney cells. TOXICOLOGY. 2024 Jun;:15387Read more>>
- Yongjian Chen. et al. Thiram exposure: Disruption of the blood-testis barrier and altered apoptosis-autophagy dynamics in testicular cells via the Bcl-2/Bax and mTOR/Atg5/p62 pathways in mice. PESTIC BIOCHEM PHYS. 2024 Aug;203:10601Read more>>
VALIDATION IMAGES