bs-0026R [Primary Antibody]
Osteopontin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Osteopontin

Immunogen Range: 221-280/294


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20750

Swiss Prot: P10923

Source: KLH conjugated synthetic peptide derived from mouse OPN

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-1 and is essential in the pathway that leads to type I immunity.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Yang, et al. "Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates." Biomaterials (2013).Read more>>
  • Zhang, Ping, et al. "Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones." Cell and Tissue Research 350.3 (2012): 425-437.Read more>>
  • Nakamura S et al. Acceleration of Bone Regeneration of Horizontal Bone Defect in Rats Using Collagen‐Binding Basic Fibroblast Growth Factor Combined with Collagen Scaffolds.J Periodontol. 2019 Mar 19. Read more>>
  • Wenchao Zhong et al. Dicalcium silicate microparticles modulate the differential expression of circRNAs and mRNAs in BMSCs and promote osteogenesis via circ_1983CmiR-6931CGas7 interaction.. Biomater Sci.2020 Jul 7;8(13):3664-3677.Read more>>
  • Qiuping Leng. et al. Demineralized bone matrix scaffold modified with mRNA derived from osteogenically pre-differentiated MSCs improves bone repair. Mat Sci Eng C-Mater. 2021 Feb;119:111601Read more>>
  • Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084Read more>>
  • Komei Kawamoto. et al. A Study of Bone Formation around Titanium Implants Using Frozen Sections. 2021 Apr 15Read more>>
  • Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359Read more>>
  • Chanjuan Dong. et al. Demineralized and decellularized bone extracellular matrix incorporated electrospun nanofibrous scaffold for bone regeneration. J Mater Chem B. 2021 AugRead more>>
  • Luo Qianting. et al. Dicalcium silicate-induced mitochondrial dysfunction and autophagy-mediated macrophagic inflammation promotes osteogenic differentiation of BMSCs. Regen Biomater. 2021 DecRead more>>
  • Hou, Yonghui. et al. Nonwoven-based gelatin/polycaprolactone membrane loaded with ERK inhibitor U0126 for treatment of tendon defects. Stem Cell Res Ther. 2022 Dec;13(1):1-11Read more>>
  • Yi Song. et al. LncRNA SENCR overexpression attenuated the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection via the miR-206/myocardin axis. Nutr Metab Cardiovas. 2022 MaRead more>>
  • Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing <i>via</i> Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393Read more>>
  • Samantha Ketelyn Silva. et al. Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation. INT J MOL SCI. 2022 Jan;23(12):6503Read more>>
  • Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683Read more>>
  • Zhenyin Chen. et al. Gelatin/sodium alginate composite hydrogel with dynamic matrix stiffening ability for bone regeneration. COMPOS PART B-ENG. 2022 Aug;243:110162Read more>>
  • Li Jianbin. et al. Characterizations of alveolar repair after mandibular second molar extraction: an experimental study in rats. J APPL ORAL SCI. 2022 Jul;3Read more>>
  • Xu Huang. et al. Osteopontin-Targeted and PPAR-Agonist-Loaded Nanoparticles Efficiently Reduce Atherosclerosis in Apolipoprotein E/ Mice. ACS OMEGA. 2022;7(33):2876728778Read more>>
  • Cao Yanyan. et al. 3D printed-electrospun PCL/Hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone. REGEN BIOMATER. 2022 DecRead more>>
  • Lin, Xiaoxin. et al. Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway. ODONTOLOGY. 2022 Dec;:1-9Read more>>
  • Sheng, Zhenxian. et al. Denervation delays initial?bone healing of rat tooth extraction socket. ODONTOLOGY. 2022 Dec;:1-9Read more>>
  • Fangyu Qiao. et al. Hybrid Cell Membrane-Functionalized Matrixes for Modulating Inflammatory Microenvironment and Improving Bone Defect Repair. ADV HEALTHC MATER. 2023 Apr;:2203047Read more>>
  • Zhenyin Chen. et al. Dynamic stiffening collagen-coated substrate enhances osteogenic differentiation of mesenchymal stem cells through integrin 21. BIOMATER SCI-UK. 2023 MayRead more>>
  • Yang S. Y.. et al. Exosomes Derived from Endothelial Cells Inhibit Neointimal Hyperplasia Induced by Carotid Artery Injury in Rats via ROS-NLRP3 Inflammasome Pathway. B EXP BIOL MED+. 2023 May;:1-6Read more>>
  • Juan Yan. et al. Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels. SCI ADV. 2023 Jul;9(29)Read more>>
  • Qinglin Ye. et al. Urinary SPP1 has potential as a non-invasive diagnostic marker for focal segmental glomerulosclerosis. FEBS OPEN BIO. 2023 SepRead more>>
  • Fancheng Xu. et al. A sequential sustained-release hydrogel with potent antimicrobial, anti-inflammatory, and osteogenesis-promoting properties for the treatment of periodontitis. CHEM ENG J. 2023 Dec;477:147195Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat shin bone labeled with Anti-Osteopontin Polyclonal Antibody, Unconjugated (bs-0026R) at 1:300 followed by conjugation to the secondary antibody and DAB staining\n


Mouse kidney lysates probed with (bs-00265R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Formalin-fixed and paraffin-embedded rat hippocampus labeled with Rabbit Anti-OPN Polyclonal Antibody, Unconjugated (bs-0026R) at 1:300 followed by conjugation to the secondary antibody


Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Osteopontin Polyclonal Antibody, Unconjugated (bs-0026R) at 1:400 overnight at 4°C, DAB staining.