bs-0023R [Primary Antibody]
GSK3B Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GSK3B

Immunogen Range: 1-50/420


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2932

Swiss Prot: P49841

Source: KLH conjugated synthetic peptide derived from human GSK3B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:500-1000)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 47


Cross Reactive Species: Mouse
Rat
Dog

Predicted Cross Reactive Species: Human
Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
  • Qingxin Fan. et al. Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/-Catenin Signaling in 3T3-L1 Adipocytes. Med Sci Monitor. 2021; 27: e928619-1Ce928619-1Read more>>
  • Liu J. et al. Moxidectin induces Cytostatic Autophagic Cell Death of Glioma Cells through inhibiting the AKT/mTOR Signalling Pathway.. J Cancer. 2020 Aug;11(19):5802-5811Read more>>
  • Zhe Wang. et al. Baicalin coadministration with lithium chloride enhanced neurogenesis via GSK3_ pathway in corticosterone induced PC-12 cells. 2022 Mar 15Read more>>
  • Yanwen Hou. et al. Prenatal PM2.5 exposure contributes to neuronal tau lesion in male offspring mice through mitochondrial dysfunction-mediated insulin resistance. ECOTOX ENVIRON SAFE. 2022 Nov;246:114151Read more>>
  • Mingjuan Yang. et al. Rosmarinic acid potentiates and detoxifies tacrine in combination for Alzheimer's disease. PHYTOMEDICINE. 2022 Dec;:1546Read more>>
  • Wang, Zhe. et al. Baicalin Ameliorates Corticosterone-Induced Depression by Promoting Neurodevelopment of Hippocampal via mTOR/GSK3 Pathway. CHIN J INTEGR MED. 2023 Jan;:1-8Read more>>
  • Huan He. et al. BDE-209 disturbed proliferation and differentiation of spermatogonia during mitotic process through estrogen receptor . REPROD BIOL. 2023 Jun;23:100737Read more>>
  • Huiqin Guo. et al. Oat -D-glucan ameliorates type II diabetes through TLR4/PI3K/AKT mediated metabolic axis. INT J BIOL MACROMOL. 2023 Jul;:126039Read more>>
  • Fan Zhu. et al. Astrocyte-secreted C3 signaling impairs neuronal development and cognition in autoimmune diseases. PROG NEUROBIOL. 2024 Jun;:102654Read more>>
  • Cuicui Zhuang. et al. Escherichia coli infection induces ferroptosis in bovine mammary epithelial cells by activating the Wnt/-catenin pathway-mediated mitophagy. MITOCHONDRION. 2024 Sep;78:101921Read more>>
VALIDATION IMAGES

Mouse Placenta lysates probed with GSK3B Polyclonal Antibody, Unconjugated bs-0023R at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


Lane 1: Mouse Muscle lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Muscle lysates probed with GSK3B Polyclonal Antibody, Unconjugated (bs-0023R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.