Host: Mouse
Target Protein: GSK-3 beta NT
Immunogen Range: 1-50/420
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2932
Swiss Prot: P49841
Source: KLH conjugated synthetic peptide derived from human GSK-3 Beta
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 47
Cross Reactive Species:
Human
Mouse
Zebrafish
Predicted Cross Reactive Species:
Rat
Dog
Cow
Sheep
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Jin Y et al. Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing -Catenin Expression. Front Pharmacol. 2020 Jun 5;11:808.Read more>>
- Peiqi Zhu. et al. Panax notoginseng saponins promote endothelial progenitor cell angiogenesis via the Wnt/-catenin pathway. Bmc Complem Altern M. 2021 Dec;21(1):1-11Read more>>
- Gao Yang. et al. HOXB5 promotes proliferation, migration, and invasion of pancreatic cancer cell through the activation of the GSK3/-catenin pathway. Anti-Cancer Drug. 2020 Sep;31(8):828-835Read more>>
- Jin Dai. et al. NK6 Homeobox 2 Regulated Gastrokin-2 Suppresses Gastric Cancer Cell Proliferation and Invasion via Akt Signaling Pathway. Cell Biochem Biophys. 2021 Mar;79(1):123-131Read more>>
- Ling Xie. et al. Suppression of GOLM1 by EGCG through HGF/HGFR/AKT/GSK-3/-catenin/c-Myc signaling pathway inhibits cell migration of MDA-MB-231. Food Chem Toxicol. 2021 Nov;157:112574Read more>>
- Shuhua Tian. et al. Sulforaphane Regulates Glucose and Lipid Metabolisms in Obese Mice by Restraining JNK and Activating Insulin and FGF21 Signal Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXXRead more>>
- Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3 via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12Read more>>
- Feng Ziqiang. et al. In Ovo Injection of CHIR-99021 Promotes Feather Follicle Development via Modulating the Wnt Signaling Pathway and Transcriptome in Goose Embryos (Anser cygnoides). FRONT PHYSIOL. 2022 May;0:811Read more>>
- Liu Jiayi. et al. Lithium Chloride Promotes Endogenous Synthesis of CLA in Bovine Mammary Epithelial Cells. BIOL TRACE ELEM RES. 2023 Apr;:1-14Read more>>
- Wei Jiang. et al. CHI3L1 signaling impairs hippocampal neurogenesis and cognitive function in autoimmune-mediated neuroinflammation. SCI ADV. 2023 Sep;9(39)Read more>>
- Jiangliu Yang. et al. Baicalin Attenuates PantonCValentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3 Pathways in Bovine Mammary Epithelial Cells. INT J MOL SCI. 2023 Jan;24(19):1452Read more>>
Mouse brain lysates probed with Mouse Anti-GSK-3 beta Polyclonal Antibody, Unconjugated (bs-0023M) at 1:200 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GSK-3 beta (NT) Polyclonal Antibody, Unconjugated (bs-0023M) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GSK-3 beta) polyclonal Antibody, Unconjugated (bs-0023M) 1:100, 90 minutes at 37°C; followed by a CY5 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (CD44v3) polyclonal Antibody, Unconjugated (bs-4497R) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.