bs-0015R [Primary Antibody]
IGF 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IGF 2

Immunogen Range: 25-51/180


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3481

Swiss Prot: P01344

Source: KLH conjugated synthetic peptide derived from human IGF II

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 7.4


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Sheep
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ma Y et al. Promotion of Insulin-like growth factor II in cell proliferation and epithelial–mesenchymal transition in hepatocellular carcinoma.J Cancer Res Ther. 2018;14(4):844-850. Read more>>
  • Lei Zhao. et al. Proteomic analysis reveals the molecular mechanism of Hippophae rhamnoides polysaccharide intervention in LPS-induced inflammation of IPEC-J2 cells in piglets. Int J Biol Macromol. 2020 Dec;164:3294Read more>>
  • Yuanyuan Wang. et al. Role of AURKA in the hypothalamusCpituitaryCtesticular axis in Tibetan sheep from Tianzhu. Gen Comp Endocr. 2021 Jan;300:113617Read more>>
  • Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096Read more>>
  • Changbin Zhao. et al. IGF2 promotes the differentiation of chicken embryonic myoblast by regulating mitochondrial remodeling. J CELL PHYSIOL. 2024 JuRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human pancreas labeled with Anti-IGF II/IGF2 Polyclonal Antibody, Unconjugated (bs-0015R), followed by conjugation to the secondary antibody and DAB staining


HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF 2 Polyclonal Antibody(bs-0015R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).