bs-0014R
[Primary Antibody]
IGF 1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: IGF 1
Immunogen Range: 49-100/195
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3479
Swiss Prot: P05019
Source: KLH conjugated synthetic peptide derived from human IGF-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IP(1-2ug)
Predicted Molecular Weight: 7.7/21
Cross Reactive Species:
Human
Mouse
Rat
Dog
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Baykara, B., et al. "Progesterone treatment decreases traumatic brain injury induced anxiety and is correlated with increased serum IGF-1 levels; prefrontal cortex, amygdala, hippocampus neuron density; and reduced serum corticosterone levels in immature rats." Biotechnic & Histochemistry 0 (2013): 1-8.Read more>>
- Kiray, M., et al. "Effects of carbon dioxide exposure on early brain development in rats." Biotechnic & Histochemistry 0 (2014): 1-13.Read more>>
- Gabr, Hala, et al. "The effect of bone marrow‐derived mesenchymal stem cells on chemotherapy induced ovarian failure in albino rats." Microscopy Research and Technique (2016).Read more>>
- Liao, Wei-Tao, et al. "The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation." (2016).Read more>>
- Müller, L., et al. "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." Reproduction in Domestic Animals (2017).Read more>>
- Uysal, Nazan, et al. "Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats." Journal of Chemical Neuroanatomy (2017).Read more>>
- Liao et al. The effect of celastrol on learning and memory in diabetic rats after sevoflurane inhalation. (2018) Arch.Med.Sci. 14:370-380Read more>>
- Muller et al. Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs. (2017) Reprod.Domest.Anim. 52 Suppl 2:187-192Read more>>
- Zhao L et al. Reducing macrophage numbers alleviates temporomandibular joint ankylosis. Cell Tissue Res. 2019 Sep 14.Read more>>
- Li R et al. Pharmacological biotargets and the molecular mechanisms of oxyresveratrol treating colorectal cancer: Network and experimental analyses. Biofactors. 2019 Oct 24.Read more>>
- Yokohira M et al. Lung proliferative lesion‐promoting effects of left pulmonary ligation in A/J female mice. Pathol Int. 2020 Feb 21. Read more>>
- Ito E et al. Food restriction reduces cortical bone mass and serum insulin-like growth factor-1 levels and promotes uterine atrophy in miceBiochem Biophys Res Commun.2021 Jan 1;534:165-171.Read more>>
- Li Taotao. et al. Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development. Front Cell Dev Biol. 2021 Mar;9:65Read more>>
- Li Taotao. et al. Unraveling Stage-Dependent Expression Patterns of Circular RNAs and Their Related ceRNA Modulation in Ovine Postnatal Testis Development. Front Cell Dev Biol. 2021 Mar;9:65Read more>>
- Qin Yang. et al. Gypenoside XLIX protects against acute kidney injury by suppressing IGFBP7/IGF1R-mediated programmed cell death and inflammation. Phytomedicine. 2021 Mar;:153541Read more>>
- Cemile Merve Seymen. et al. Involvement of endometrial IGF-1R/IGF-1/Bcl-2 pathways in experimental polycystic ovary syndrome: Identification of the regulatory effect of melatonin. Tissue Cell. 2021 Jun;:101585Read more>>
- XuTing Song. et al. Molecular cloning, expression, and functional features of IGF1 splice variants in sheep. Endocr Connect. 2021 Jul;-1(aop)Read more>>
- Yongfu La. et al. Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak. 2021 Nov 09Read more>>
- Qinqin Hu. et al. An injectable thermosensitive Pluronic F127/hyaluronic acid hydrogel loaded with human umbilical cord mesenchymal stem cells and asiaticoside microspheres for uterine scar repair. INT J BIOL MACROMOL. 2022 JulRead more>>
- Yue Wang. et al. Potential use of bioactive nanofibrous dural substitutes with controlled release of IGF-1 for neuroprotection after traumatic brain injury. NANOSCALE. 2022 NovRead more>>
- Akihito Sotome. et al. Spinal Canal and Spinal Cord in Rat Continue to Grow Even after Sexual Maturation: Anatomical Study and Molecular Proposition. INT J MOL SCI. 2022 Jan;23(24):16076Read more>>
- Nevin Ersoy. et al. The Effects of Prenatal and Postnatal Exposure to 50-Hz and 3 mT Electromagnetic Field on Rat Testicular Development. MED LITH. 2023 Jan;59(1):71Read more>>
- Yingchao Shen. et al. Proteomic differences between the ovulatory and anovulatory sides of the mare's follicular and oviduct fluid. J EQUINE VET SCI. 2022 Dec;:104207Read more>>
- Qiyu Gan. et al. Prognostic Value and Immune Infiltration of HPV-Related Genes in the Immune Microenvironment of Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma. CANCERS. 2023 Jan;15(5):1419Read more>>
- Man Luo. et al. miR136 regulates proliferation and differentiation of small tail han sheep preadipocytes. ADIPOCYTE. 2023;12(1):Article: 2173966Read more>>
- Ryo Honma. et al. Immunomodulatory Macrophages Enable E-MNC Therapy for Radiation-Induced Salivary Gland Hypofunction. CELLS-BASEL. 2023 Jan;12(10):1417Read more>>
- Yang Yuying. et al. Exploring the potential mechanism of Xiaojin Pill therapy for benign prostatic hyperplasia through metabolomics and gut microbiota analysis. FRONT MICROBIOL. 2024 Aug;15Read more>>
VALIDATION IMAGES
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT.
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4°C, DAB staining.