bs-0009R [Primary Antibody]
alpha Synuclein Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: alpha Synuclein

Immunogen Range: 51-90/140


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6622

Swiss Prot: P37840

Source: KLH conjugated synthetic peptide derived from human alpha Synuclein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 14


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Danmin Lin. et al. Brain-targeted gene delivery of ZnO quantum dots nanoplatform for the treatment of Parkinson disease. Chem Eng J. 2022 Feb;429:13221Read more>>
  • Yifei Gao. et al. NIR-Assisted MgO-Based Polydopamine Nanoparticles for Targeted Treatment of Parkinson's Disease Through the Blood-Brain Barrier. ADV HEALTHC MATER. 2022 Sep;:2201655Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-alpha-Synuclein (NT) Polyclonal Antibody, Unconjugated (bs-0009R) 1:300 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0009R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (alpha-Synuclein) polyclonal Antibody, Unconjugated (bs-0009R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.