bs-0008R
[Primary Antibody]
Nestin Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Nestin
Immunogen Range: 681-750/1893
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 25491
Swiss Prot: P21263
Source: KLH conjugated synthetic peptide derived from rat Nestin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 209
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yamamizu, Kohei, et al. "Protein Kinase A Determines Timing of Early Differentiation through Epigenetic Regulation with G9a." Cell stem cell 10.6 (2012): 759-770.Read more>>
- Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456.Read more>>
- Shan, Tengfei, et al. "Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β‐catenin signaling." Cell Biology International (2014).Read more>>
- Kawashima, Satoki, Koichi SHINKAI, and Masaya SUZUKI. "Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping." Dental Materials Journal 35.3 (2016): 479-489.Read more>>
- Wang et al. Formylpeptide Receptors Promote the Migration and Differentiation of Rat Neural Stem Cells. (2016) Sci.Re. 6:25946Read more>>
- Sandikci, M. et al. “Changes on the Pancreas in Experimental Diabetes and the Effect of Lycopene on These Changes: Pdx‐1, Ngn‐3, and Nestin Expressions.” The Anatomical Record (2017)Read more>>
- Yu D et al. MOF-encapsulated nanozyme enhanced siRNA combo: Control neural stem cell differentiation and ameliorate cognitive impairments in Alzheimer's disease model. Biomaterials. 2020 Oct;255:120160.Read more>>
- Xue Wang. et al. Engineered liposomes targeting the gutCCNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:39Read more>>
- Yong Du. et al. Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development. Med Sci Monitor. 2020; 26: e927334-1Ce927334-1Read more>>
- Mengna Duan. et al. Epidermal stem cell-derived exosomes promote skin regeneration by downregulating transforming growth factor-1 in wound healing. Stem Cell Res Ther. 2020 Dec;11(1):1-11Read more>>
- Fumiaki Sato. et al. Pulp tissue reaction to a self-adhesive, resin-based direct pulp capping material containing surface pre-reacted glass-ionomer filler. Dent Mater. 2021 MaRead more>>
- Lu Hong. et al. Investigation of Naoluoxintong on the neural stem cells by facilitating proliferation and differentiation in vitro and on protecting neurons by up-regulating the expression of nestin in MCAO rats. J ETHNOPHARMACOL. 2022 Sep;:115684Read more>>
- K?KKAYA EREN Selen. et al. Combined effects of bone morphogenetic protein-7 and mineral trioxide aggregate on the proliferation, migration, and differentiation of human dental pulp stem cells. J APPL ORAL SCI. 2022 Sep;3Read more>>
- Ma Caiyun. et al. In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells. BRAZ ARCH BIOL TECHN. 2023 Feb;66Read more>>
- Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936Read more>>
- Hao Wu. et al. Nattokinase Promotes Post-stroke Neurogenesis and Cognition Recovery via Increasing Circulating Irisin. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
- Hu Pengfei. et al. Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler. CELL MOL BIOL LETT. 2024 Dec;29(1):1-19Read more>>
- Hongjuan Wang. et al. Differentiation of cultured hair follicle neural crest stem cells into functional melanocytes. HELIYON. 2024 Aug;1Read more>>
VALIDATION IMAGES
Neuro-2a cells probed with Rabbit Anti-Nestin Polyclonal Antibody (bs-0008R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Nestin Polyclonal Antibody (bs-0008R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat hippocampus labeled with Anti-Nestin Polyclonal Antibody (bs-0008R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
Tissue/cell:U-251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nestin) polyclonal Antibody, Unconjugated (bs-0008R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.